Gold–silver-nanoclusters having cholic acid imprinted nanoshell

• Published in: Talanta (Oxford) 2012-05, Vol.93, p.364-370
• Main Authors: Gültekin, Aytaç, Ersöz, Arzu, Denizli, Adil, Say, Rıdvan
• Format: Article
• Language: English
• Subjects: Affinity; Analytical chemistry; Binding; Chemistry; Cholic acid; Cholic Acid - analysis; Cholic Acid - blood; Cholic Acid - chemistry; Cholic Acid - urine; Cysteine - chemistry; Exact sciences and technology; General, instrumentation; Gold - chemistry; Gold–silver-nanoclusters; Holes; Humans; Luminescent Measurements; Metal Nanoparticles - chemistry; Molecular Imprinting; Molecularly imprinted polymers; Nanocomposites; Nanomaterials; Nanostructure; Photoluminesence; Polymerization; Recognition; Sensors; Silver - chemistry; Sulfhydryl Compounds - chemistry
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2012.02.057

► Molecular imprinting is a technology to create recognition sites in a macromolecular matrix. ► MIPs are easy to prepare, stable, inexpensive and capable of molecular recognition. ► A nanocluster is an amorphous/semicrystalline nanostructure. ► We have combined nanoscale materials with MIP. Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP-nanoparticles have started to appear in the literature. In this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamido-cysteine (MAC) attached to gold–silver-nanoclusters reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for cholic acid recognition. In this method, methacryloylamidohistidine–Pt(II) [MAH–Pt(II)] has used as a new metal-chelating monomer via metal coordination–chelation interactions and cholic acid. Nanoshell sensors with templates give a cavity that is selective for cholic acid. The cholic acid can simultaneously chelate to Pt(II) metal ion and fit into the shape-selective cavity. Thus, the interaction between Pt(II) ion and free coordination spheres has an effect on the binding ability of the gold–silver-nanoclusters nanosensor. The binding affinity of the cholic acid imprinted nanoparticles have investigated by using the Langmuir and Scatchard methods and determined affinity constant (Kaffinity) has found to be 2.73×104molL−1 and 2.13×108molL−1, respectively. At the last step of this procedure, cholic acid level in blood serum and urine which belong to a healthy people were determined by the prepared gold–silver-nanoclusters.