Cutaneous Uptake of 14C-Hd Vapor By The Hairless Guinea Pig

• Published in: Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 1999-05, Vol.22 (2), p.375-387
• Main Authors: Logan, Thomas P., Millard, Charles B., Shutz, Michael, Schulz, Susan M., Lee, Robyn B., Bongiovanni, Rodolfo
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Carbon Radioisotopes; Carcinogens - pharmacokinetics; Carcinogens - toxicity; Chemical and industrial products toxicology. Toxic occupational diseases; Chemical Warfare Agents - pharmacokinetics; Chemical Warfare Agents - toxicity; Dermatologic Agents - pharmacokinetics; Dermatologic Agents - toxicity; Gas, fumes; Gases; Guinea Pigs; Male; Medical sciences; Mustard Gas - pharmacokinetics; Mustard Gas - toxicity; Skin - metabolism; Skin Absorption - drug effects; Toxicology
• ISSN: 0148-0545; 1525-6014
• DOI: 10.3109/01480549909017841

ABSTRACT The hairless guinea pig (HGP) is used by our laboratory to model the human cutaneous response to sulfur mustard (HD), bis(2-chloroethylsulfide), exposure. We determined the HD content in the skin of HGP after a 7-min exposure to vapors saturated with a mixture of HD and l4C-HD. Concentration/time (CT) values in the range of 2 μg/cm2/min were determined by counting skin 14C disintegrations per min (dpm) in animals euthanized immediately after exposure. These values are similar to human penetration rates obtained by other investigators. A rate curve monitoring the reduction in skin 14C dpm was developed for animals euthanized between 0 and 24 hr post- exposure. This curve showed the greatest change after 1 hr. The epidermal (62%) to dermal (38%) ratio of 14C at 24 hr was measured for two animals. We saw no site preference for HD penetration among the 8 sites used. The 14C content of template adhesive tape was determined to follow HD distribution. These results contribute to a better understanding of the cutaneous response to HD in the HGP model.