Ethanol Oxidation and Acetaldehyde Production in Vitro by Human Intestinal Strains of Escherichia coli under Aerobic, Microaerobic, and Anaerobic Conditions

Background: Many human colonic facultative anaerobic and aerobic bacteria are capable of alcohol dehydrogenase (ADH)-mediated ethanol oxidation. In this bacteriocolonic pathway for ethanol oxidation intracolonic ethanol is first oxidized by bacterial ADHs to acetaldehyde, which is further oxidized b...

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Published in:Scandinavian journal of gastroenterology 1999, Vol.34 (10), p.967-973
Main Authors: SALASPURO, V, NYFORS, S, HEINE, R, SIITONEN, A, SALASPURO, M, JOUSIMIES-SOMER, H
Format: Article
Language:English
Subjects:
Acetaldehyde - metabolism
Alcohol Dehydrogenase - metabolism
Bacteria, Aerobic - enzymology
Bacteria, Aerobic - physiology
Bacteria, Anaerobic - enzymology
Bacteria, Anaerobic - physiology
Bacteriology
Biological and medical sciences
Enterobacteriaceae - enzymology
Escherichia coli - enzymology
Ethanol - metabolism
Fermentation - physiology
Fundamental and applied biological sciences. Psychology
Humans
Intestinal Mucosa - microbiology
Intestinal Mucosa - physiology
Metabolism. Enzymes
Microbiology
Oxidation-Reduction
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Summary:Background: Many human colonic facultative anaerobic and aerobic bacteria are capable of alcohol dehydrogenase (ADH)-mediated ethanol oxidation. In this bacteriocolonic pathway for ethanol oxidation intracolonic ethanol is first oxidized by bacterial ADHs to acetaldehyde, which is further oxidized by either colonic mucosal or bacterial aldehyde dehydrogenases to acetate. The produced acetaldehyde is a highly toxic and carcinogenic agent. This study was aimed to investigate the ethanol oxidation capability and acetaldehyde formation of Escherichia coli IH 50546 and IH 50817. These intestinal E. coli strains expressed either high (IH 50546) or low (IH 50817) ADH activity. Methods: Strains were cultured for 48 h on agar plates supplemented with ethanol under aerobic, microaerobic (6% O2), and anaerobic conditions. Results: Under aerobic conditions both E. coli
ISSN:0036-5521
1502-7708
DOI:10.1080/003655299750025057