Analysis of radiation-induced chromosomal aberrations using telomeric and centromeric PNA probes

Purpose : To generate dose-response curves for X-ray-induced chromosomal aberrations analysed in human blood lymphocytes using telomeric and centromeric peptide nucleic acid (PNA) probes. Materials and methods : Isolated human lymphocytes were X-irradiated with doses of 0, 1, 2, 3, 4 and 6 Gy. Aberr...

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Bibliographic Details
Published in:International journal of radiation biology 2000, Vol.76 (2), p.163-167
Main Authors: BOEI, J. J. W. A, VERMEULEN, S, NATARAJAN, A. T
Format: Article
Language:English
Subjects:
Biological and medical sciences
Centromere
Chromatin. Chromosome
Chromosome Aberrations
Dose-Response Relationship, Radiation
Fundamental and applied biological sciences. Psychology
Humans
Lymphocytes - radiation effects
Lymphocytes - ultrastructure
Molecular and cellular biology
Molecular genetics
Nucleic Acid Probes
Telomere
X-Rays
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Summary:Purpose : To generate dose-response curves for X-ray-induced chromosomal aberrations analysed in human blood lymphocytes using telomeric and centromeric peptide nucleic acid (PNA) probes. Materials and methods : Isolated human lymphocytes were X-irradiated with doses of 0, 1, 2, 3, 4 and 6 Gy. Aberrations were analysed in the first post-irradiation metaphases using telomeric and centromeric PNA probes. Results : Similar to the dose-response curves for the yield of dicentrics and centric rings, the dose-response curves for interstitial fragments and incomplete elements (derived from either terminal deletions or incomplete exchanges) follow a linear-quadratic function. Furthermore, it was estimated that 76% of excess acentric fragments originate from complete exchanges (interstitial deletions) and only 24% from incomplete exchanges or terminal deletions. Conclusions : Interstitial fragments form a major class of radiation-induced chromosomal aberrations. They are induced about half as frequently as dicentrics over the whole dose range investigated. The comparable trend of the dose-response curve for the different aberrations, including incomplete elements, indicates that all detected aberrations are formed by a similar underlying mechanism. It also suggests that the ratio between non- or incomplete repair (leading to open ends of broken chromosomes) and incorrect repair (leading to exchange aberrations) is independent of dose.
ISSN:0955-3002
1362-3095
DOI:10.1080/095530000138817