IL-10 reduces Th2 cytokine production and eosinophilia but augments airway reactivity in allergic mice

Departments of 1  Physiology, 2  Comparative Medicine, and 3  Medicine, East Carolina University, Greenville, North Carolina 27858; and 4  Department of Internal Medicine, Section of Allergy and Immunology, University of Texas Medical Branch, Galveston, Texas 77555 We investigated the effects of int...

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Published in:American journal of physiology. Lung cellular and molecular physiology 2000-04, Vol.278 (4), p.667-L674
Main Authors: van Scott, Michael R, Justice, J. Paul, Bradfield, John F, Enright, Edward, Sigounas, Anastasia, Sur, Sanjiv
Format: Article
Language:English
Subjects:
AIDS/HIV
Animals
Bronchial Hyperreactivity - physiopathology
Bronchitis - pathology
Bronchoalveolar Lavage Fluid - chemistry
Cytokines - antagonists & inhibitors
Cytokines - biosynthesis
Cytokines - secretion
Dose-Response Relationship, Drug
Eosinophilia - pathology
Female
Hypersensitivity - metabolism
Hypersensitivity - pathology
Hypersensitivity - physiopathology
Interleukin-10 - administration & dosage
Interleukin-10 - analysis
Interleukin-10 - blood
Interleukin-10 - pharmacology
Methacholine Chloride
Mice
Mice, Inbred BALB C
Pollen - immunology
Th2 Cells - metabolism
Trachea
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Summary:Departments of 1  Physiology, 2  Comparative Medicine, and 3  Medicine, East Carolina University, Greenville, North Carolina 27858; and 4  Department of Internal Medicine, Section of Allergy and Immunology, University of Texas Medical Branch, Galveston, Texas 77555 We investigated the effects of interleukin (IL)-10 administration on allergen-induced Th2 cytokine production, eosinophilic inflammation, and airway reactivity. Mice were sensitized by intraperitoneal injection of ragweed (RW) adsorbed to Alum and challenged by intratracheal instillation of the allergen. Sensitization and challenge with RW increased concentrations of IL-10 in bronchoalveolar lavage (BAL) fluid from undetectable levels to 60 pg/ml over 72 h. Intratracheal instillation of 25 ng of recombinant murine IL-10 at the time of RW challenge further elevated BAL fluid IL-10 concentration to 440 pg/ml but decreased BAL fluid IL-4, IL-5, and interferon- levels by 40-85% and eosinophil numbers by 70% ( P  
ISSN:1040-0605
1522-1504
DOI:10.1152/ajplung.2000.278.4.l667