Use of an immunoassay as a means to detect polychlorinated biphenyls in animal fat

In this paper we describe the validation and utilization of the Hybrizyme™ DELFIA® immunoassay for detecting polychlorinated biphenyls (PCBs) in animal fat. The immunoassay incorporates a rapid sample processing protocol that has been optimized to detect concentrations of PCBs in animal fat at or ab...

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Bibliographic Details
Published in:Food additives and contaminants 2001-02, Vol.18 (2), p.121-127
Main Authors: Jaborek-Hugo, S., Von Holst, C., Allen, R., Stewart, T., Willey, J., Anklam, E.
Format: Article
Language:English
Subjects:
Adipose Tissue - chemistry
Animals
Biological and medical sciences
Calibration
False Negative Reactions
False Positive Reactions
Fat industries
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
General aspects
Immunoassay - methods
Meat - analysis
Medical sciences
Methods of analysis, processing and quality control, regulation, standards
Pesticides, fertilizers and other agrochemicals toxicology
Polychlorinated Biphenyls - analysis
Polychlorinated Biphenyls Delfia Immunoassay Animal Fat Screening Method
Swine
Toxicology
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Summary:In this paper we describe the validation and utilization of the Hybrizyme™ DELFIA® immunoassay for detecting polychlorinated biphenyls (PCBs) in animal fat. The immunoassay incorporates a rapid sample processing protocol that has been optimized to detect concentrations of PCBs in animal fat at or above 200ng/g. The limit of detection of the assay was less than 50ng/g. When the action level for the DELFIA assay was set at 100ng/g to detect samples having 200ng/g or more PCBs, the false negative rate was estimated at 0.2%. The false positive rate depends on the concentration of PCBs in the sample population and was estimated at 4% and 7% for samples having 10ng/ g and 20ng/g PCBs, respectively. By utilizing the DELFIA assay system to select for positive samples and GC/MS analysis for confirmation analysis, an accurate and cost-effective programme has been established for the high throughput detection of PCBs in animal products.
ISSN:0265-203X
1464-5122
DOI:10.1080/02652030010021422