A new method for encapsulation of living cells: preliminary results with PC12 cell line

A new method is described for encapsulation of living cells. PC12 rat adrenal pheochromocytoma cells, which have been shown to synthesize, store and release dopamine were employed. The particles are made first and the cells then incorporated in a gentle mechanical procedure. The morphology (by light...

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Bibliographic Details
Published in:Journal of microencapsulation 2001-05, Vol.18 (3), p.323-334
Main Authors: MERCIER, Ph, FERNANDEZ, F, TORTOSA, F, BAGHERI, H, DUPLAN, H, TAFANI, M, BES, J. C, BASTIDE, R, LAZORTHES, Y, SALLERIN, B
Format: Article
Language:English
Subjects:
Animals
Biodegradation, Environmental
Biological and medical sciences
Cell Division
Cell Survival
Cells, Immobilized
Dopamine - secretion
Drug Compounding - methods
General pharmacology
Humans
Immunohistochemistry
Medical sciences
Microencapsulation Pc12 Cell Line Dopamine
Microscopy, Electron, Scanning
Microspheres
Particle Size
PC12 Cells
Permeability
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Rats
Time Factors
Tyrosine 3-Monooxygenase - metabolism
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Summary:A new method is described for encapsulation of living cells. PC12 rat adrenal pheochromocytoma cells, which have been shown to synthesize, store and release dopamine were employed. The particles are made first and the cells then incorporated in a gentle mechanical procedure. The morphology (by light and electron microscopic observation), stability, rheology, texture and permeability of these microcapsules provided by Kappa Biotech were investigated. Membrane permeability studies demonstrated exclusion of 69000 Da human serum albumin, but equilibrium of D-glucose and inulin was within 24 h, indicating a molecular weight cut-off in the 5000-70000 Da range. The viability and the function of the encapsulated cells were evaluated by measuring the spontaneous release of dopamine by high performance liquid chromatography with electrochemical detection. The results show that dopamine-secreting cells can be sequestered in a semi-permeable capsule and still display good viability and proliferation for at least 1 month.
ISSN:0265-2048
1464-5246
DOI:10.1080/02652040010018092