The Lassa Virus Glycoprotein Precursor GP-C is Proteolytically Processed by Subtilase SKI-1/S1P

The surface glycoprotein of the Lassa virus, a member of the arenaviridae family, is synthesized as a 76-kDa precursor (GP-C) that is posttranslationally cleaved into an N-terminal 44-kDa subunit and a C-terminal membrane-anchored 36-kDa subunit. Cleavage occurs at the C-terminal end of the unusual...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2001-10, Vol.98 (22), p.12701-12705
Main Authors: Lenz, Oliver, Meulen, Jan ter, Klenk, Hans-Dieter, Seidah, Nabil G., Garten, Wolfgang
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Calcimycin - pharmacology
Calcium
Cercopithecus aethiops
Chick Embryo
CHO Cells
Cricetinae
Enzymes
Glycoproteins
Infections
Lassa virus
Lassa virus - drug effects
Lassa virus - metabolism
Membranes
Microbiology
Proprotein Convertases
Protein Precursors - metabolism
Proteins
Serine Endopeptidases - physiology
subtilase
Transfection
Vero Cells
Viral Envelope Proteins - metabolism
Virions
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The surface glycoprotein of the Lassa virus, a member of the arenaviridae family, is synthesized as a 76-kDa precursor (GP-C) that is posttranslationally cleaved into an N-terminal 44-kDa subunit and a C-terminal membrane-anchored 36-kDa subunit. Cleavage occurs at the C-terminal end of the unusual recognition motif R-R-L-L. We show here that GP-C is cleaved in the endoplasmic reticulum by the cellular subtilase SKI-1/S1P, an enzyme that has so far been observed to be involved in cholesterol metabolism. Furthermore, we present evidence that only cleaved glycoprotein is incorporated into virions and that this is necessary for the formation of infectious virus. To our knowledge, there have been no previous reports of this type of viral glycoprotein processing, one that may be an interesting target for antiviral therapy.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.221447598