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Peripheral urocortin inhibits gastric emptying and food intake in mice: differential role of CRF receptor 2
1 Center for Ulcer Research and Education: Digestive Diseases Research Center, Veterans Affairs Greater Los Angeles Healthcare System, Department of Medicine, Division of Digestive Diseases and Brain Research Institute, University of California at Los Angeles, Los Angeles 90073; 3 Clayton Foundati...
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Published in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2001-11, Vol.281 (5), p.1401-R1410 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
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Online Access: | Get full text |
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Summary: | 1 Center for Ulcer Research and Education: Digestive
Diseases Research Center, Veterans Affairs Greater Los Angeles
Healthcare System, Department of Medicine, Division of Digestive
Diseases and Brain Research Institute, University of California at Los
Angeles, Los Angeles 90073; 3 Clayton Foundation
Laboratories for Peptide Biology, The Salk Institute, La
Jolla, California 92037; and 2 Cardenal Herrera University,
Department of Basic Biomedical Sciences, 46113 Moncada, Valencia,
Spain
Intraperitoneal urocortin
inhibits gastric emptying and food intake in mice. We investigated
corticotropin-releasing factor receptor (CRF-R) subtypes involved in
intraperitoneal urocortin actions using selective CRF-R antagonists.
Gastric emptying was measured 2 h after a chow meal, and food
intake was measured hourly after an 18-h fast in mice.
Urocortin (3 µg/kg ip) inhibited gastric emptying by 88%. The
CRF-R1/CRF-R2 antagonist astressin B (30 µg/kg ip) and the selective
CRF-R2 antagonist antisauvagine-30 (100 µg/kg ip) completely
antagonized urocortin action, whereas the selective CRF-R1 antagonist
CP-154,526 (10 mg/kg ip) had no effect. Urocortin (1-10 µg/kg
ip) dose dependently decreased the 2-h cumulative food intake by
30-62%. Urocortin (3 µg/kg)-induced hypophagia was completely
antagonized by astressin B (30 µg/kg ip) and partially (35 and 31%)
by antisauvagine-30 (100 or 200 µg/kg ip). The CRF-R1 antagonists
CP-154,526 or DMP904 (10 mg/kg ip) had no effect. Capsaicin did not
alter urocortin-inhibitory actions while blocking the satiety effect of
intraperitoneal CCK. These data indicate that intraperitoneal
urocortin-induced decrease in feeding is only partly mediated by
CRF-R2, whereas urocortin action to delay gastric emptying of a meal
involves primarily CRF-R2.
antisauvagine-30; astressin B; capsaicin; cholecystokinin; CP-154,526; DMP904; corticotropin-releasing factor |
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ISSN: | 0363-6119 1522-1490 |
DOI: | 10.1152/ajpregu.2001.281.5.r1401 |