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Electrical stimulation of transforming growth factor-beta 1 secretion by human dermal fibroblasts and the U937 human monocytic cell line
The in vitro effects on human dermal fibroblasts and the U937 human monocytic cell line of three phases of electrical microcurrents generated by the ACE Stimulator were investigated. The growth and viability of growing and confluent dermal fibroblasts were not directly influenced by the separate mic...
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| Published in: | Alternatives to laboratory animals 2001-11, Vol.29 (6), p.693 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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| container_issue | 6 |
| container_start_page | 693 |
| container_title | Alternatives to laboratory animals |
| container_volume | 29 |
| creator | Todd, I Clothier, R H Huggins, M L Patel, N Searle, K C Jeyarajah, S Pradel, L Lacey, K L |
| description | The in vitro effects on human dermal fibroblasts and the U937 human monocytic cell line of three phases of electrical microcurrents generated by the ACE Stimulator were investigated. The growth and viability of growing and confluent dermal fibroblasts were not directly influenced by the separate microcurrent phases. One form of microcurrent (designated phase 1) stimulated both dermal fibroblasts and U937 cells to secrete transforming growth factor-beta 1 (TGF-beta 1), which is an important regulator of cell-mediated inflammation and tissue regeneration, but none of the three phases stimulated secretion of the pro-inflammatory cytokine interleukin-6 by U937 cells. The stimulation of TGF-beta 1 secretion in these experiments was not dramatic (a median increase over control levels of 20-30%), although it could be biologically significant. |
| doi_str_mv | 10.1177/026119290102900611 |
| format | article |
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The stimulation of TGF-beta 1 secretion in these experiments was not dramatic (a median increase over control levels of 20-30%), although it could be biologically significant.</description><subject>Animal Testing Alternatives</subject><subject>Cell Survival</subject><subject>Dermis - cytology</subject><subject>Dermis - drug effects</subject><subject>Dermis - metabolism</subject><subject>Electric Stimulation - adverse effects</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>Interleukin-6 - metabolism</subject><subject>Mitochondria - metabolism</subject><subject>Monocytes - cytology</subject><subject>Monocytes - drug effects</subject><subject>Monocytes - metabolism</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Transforming Growth Factor beta - metabolism</subject><subject>Transforming Growth Factor beta1</subject><subject>U937 Cells</subject><issn>0261-1929</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNo1kM1OAyEURllobK2-gAvDC4xyB2aQpWmqNWnixq4bYKDF8NMAjekb-NhOtW7uzT0591t8CN0BeQDg_JG0PYBoBQEyDjIeF2h6gs2JTtB1KZ8jZhTEFZqML0QQRqfoe-GNrtlp6XGpLhy8rC5FnCyuWcZiUw4ubvE2p6-6w1bqmnKjTJUYcDE6m19dHfHuEGTEg8lhjLJO5aS8LLVgGQdcdwavBeVnK6SY9LE6jbXxHnsXzQ26tNIXc3veM7R-WXzMl83q_fVt_rxq9kBFbYTQygxtZ3puBe1Yp6EjmglONciWa8Y70OpJEat6AmDBSjaaihHWU94TOkP3f7n7gwpm2OyzCzIfN_-V0B_h8mN0</recordid><startdate>200111</startdate><enddate>200111</enddate><creator>Todd, I</creator><creator>Clothier, R H</creator><creator>Huggins, M L</creator><creator>Patel, N</creator><creator>Searle, K C</creator><creator>Jeyarajah, S</creator><creator>Pradel, L</creator><creator>Lacey, K L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>200111</creationdate><title>Electrical stimulation of transforming growth factor-beta 1 secretion by human dermal fibroblasts and the U937 human monocytic cell line</title><author>Todd, I ; Clothier, R H ; Huggins, M L ; Patel, N ; Searle, K C ; Jeyarajah, S ; Pradel, L ; Lacey, K L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p139t-99cbed25e67f93545c150c4973c1a27c4751cb8b0fb6011f1fa47f9b404637603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animal Testing Alternatives</topic><topic>Cell Survival</topic><topic>Dermis - cytology</topic><topic>Dermis - drug effects</topic><topic>Dermis - metabolism</topic><topic>Electric Stimulation - adverse effects</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Humans</topic><topic>Interleukin-6 - metabolism</topic><topic>Mitochondria - metabolism</topic><topic>Monocytes - cytology</topic><topic>Monocytes - drug effects</topic><topic>Monocytes - metabolism</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Transforming Growth Factor beta - metabolism</topic><topic>Transforming Growth Factor beta1</topic><topic>U937 Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Todd, I</creatorcontrib><creatorcontrib>Clothier, R H</creatorcontrib><creatorcontrib>Huggins, M L</creatorcontrib><creatorcontrib>Patel, N</creatorcontrib><creatorcontrib>Searle, K C</creatorcontrib><creatorcontrib>Jeyarajah, S</creatorcontrib><creatorcontrib>Pradel, L</creatorcontrib><creatorcontrib>Lacey, K L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Alternatives to laboratory animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Todd, I</au><au>Clothier, R H</au><au>Huggins, M L</au><au>Patel, N</au><au>Searle, K C</au><au>Jeyarajah, S</au><au>Pradel, L</au><au>Lacey, K L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrical stimulation of transforming growth factor-beta 1 secretion by human dermal fibroblasts and the U937 human monocytic cell line</atitle><jtitle>Alternatives to laboratory animals</jtitle><addtitle>Altern Lab Anim</addtitle><date>2001-11</date><risdate>2001</risdate><volume>29</volume><issue>6</issue><spage>693</spage><pages>693-</pages><issn>0261-1929</issn><abstract>The in vitro effects on human dermal fibroblasts and the U937 human monocytic cell line of three phases of electrical microcurrents generated by the ACE Stimulator were investigated. 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| identifier | ISSN: 0261-1929 |
| ispartof | Alternatives to laboratory animals, 2001-11, Vol.29 (6), p.693 |
| issn | 0261-1929 |
| language | eng |
| recordid | cdi_pubmed_primary_11709043 |
| source | Sage Journals Online |
| subjects | Animal Testing Alternatives Cell Survival Dermis - cytology Dermis - drug effects Dermis - metabolism Electric Stimulation - adverse effects Enzyme-Linked Immunosorbent Assay Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - metabolism Humans Interleukin-6 - metabolism Mitochondria - metabolism Monocytes - cytology Monocytes - drug effects Monocytes - metabolism Tetradecanoylphorbol Acetate - pharmacology Transforming Growth Factor beta - metabolism Transforming Growth Factor beta1 U937 Cells |
| title | Electrical stimulation of transforming growth factor-beta 1 secretion by human dermal fibroblasts and the U937 human monocytic cell line |
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