Normovolemic hemodilution with Hb vesicle solution attenuates hypoxia in ischemic hamster flap tissue

1  Department of Orthopedic, Plastic, and Hand Surgery, Inselspital University Hospital, 3010 Berne, Switzerland; and 2  Advanced Research Institute for Science and Engineering, Waseda University, Tokyo 169-8555, Japan The aim of this study was to test whether oxygenation in acutely ischemic, collat...

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Published in:American journal of physiology. Heart and circulatory physiology 2003-05, Vol.284 (5), p.H1702-H1709
Main Authors: Erni, Dominique, Wettstein, Reto, Schramm, Soren, Contaldo, Claudio, Sakai, Hiromi, Takeoka, Shinji, Tsuchida, Eishun, Leunig, Michael, Banic, Andrej
Format: Article
Language:English
Subjects:
Animals
Blood Substitutes - pharmacology
Collateral Circulation - physiology
Cricetinae
Hemodilution - methods
Hemoglobins - pharmacology
Hypoxia - therapy
Liposomes - pharmacology
Male
Mesocricetus
Microcirculation - physiology
Microscopy - methods
Oxygen - blood
Surgical Flaps - blood supply
Surgical Flaps - physiology
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Summary:1  Department of Orthopedic, Plastic, and Hand Surgery, Inselspital University Hospital, 3010 Berne, Switzerland; and 2  Advanced Research Institute for Science and Engineering, Waseda University, Tokyo 169-8555, Japan The aim of this study was to test whether oxygenation in acutely ischemic, collateralized tissue may be improved by normovolemic hemodilution with a solution containing liposome-encapsulated human Hb (HbV). A skin flap model in anesthetized hamsters was used, which consisted of two parts receiving either anatomic or collateral perfusion. Microhemodynamics were investigated with intravital microscopy. Partial tissue oxygen tension was measured with a Clark-type microprobe. Hemodilution was obtained by exchanging 50% of the total blood volume with HbV suspended in 8% human serum albumin (HSA8) or 6% Dextran 70 (Dx70). The size of the vesicles was 276 nm, the P 50 was 22 mmHg, and the Hb concentration of the solutions was 7.5 g/dl. Colloid osmotic pressure and viscosity were 49.9 mmHg and 8.7 cP for HbV-Dx70 and 40.0 mmHg and 2.9 cP for HbV-HSA8, respectively. Hemodilution with HbV-Dx70 led to an increase in microvascular blood flow in the ischemic microvessels to maximally 158% (median, P  
ISSN:0363-6135
1522-1539
DOI:10.1152/ajpheart.00821.2002