Molecular phenotype of airway side population cells

Departments of 1 Environmental and Occupational Health, 2 Cell Biology and Physiology, and 3 Radiation Oncology, University of Pittsburgh, Pittsburgh, Pennsylvania 15260 Submitted 14 May 2003 ; accepted in final form 30 July 2003 Lung epithelial-specific stem cells have been localized to discrete mi...

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Bibliographic Details
Published in:American journal of physiology. Lung cellular and molecular physiology 2004-04, Vol.286 (4), p.624-L630
Main Authors: Giangreco, Adam, Shen, Hongmei, Reynolds, Susan D, Stripp, Barry R
Format: Article
Language:English
Subjects:
Animals
Antigens, Ly - analysis
Benzimidazoles
Cell Separation
Epithelial Cells - cytology
Fluorescent Dyes
Gene Expression
Hematopoietic Stem Cells - chemistry
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - physiology
Leukocyte Common Antigens - analysis
Lung - cytology
Membrane Proteins - analysis
Mice
Mice, Inbred Strains
Pancreatic Elastase
Phenotype
Proteins - genetics
Pulmonary Surfactant-Associated Protein C - genetics
Uteroglobin
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Summary:Departments of 1 Environmental and Occupational Health, 2 Cell Biology and Physiology, and 3 Radiation Oncology, University of Pittsburgh, Pittsburgh, Pennsylvania 15260 Submitted 14 May 2003 ; accepted in final form 30 July 2003 Lung epithelial-specific stem cells have been localized to discrete microenvironments throughout the adult conducting airway. Properties of these cells include pollutant resistance, multipotent differentiation, and infrequent proliferation. Goals of the present study were to use Hoechst 33342 efflux, a property of stem cells in other tissues, to purify and further characterize airway stem cells. Hoechst 33342 effluxing lung cells were identified as a verapamil-sensitive side population by flow cytometry. Lung side population cells were further subdivided on the basis of hematopoietic (CD45 positive) or nonhematopoietic (CD45 negative) origin. Nonhematopoietic side population cells were enriched for stem cell antigen-1 reactivity and expressed molecular markers specific to both airway and mesenchymal lineages. Analysis of the molecular phenotype of airway-derived side population cells indicates that they are similar to neuroepithelial body-associated variant Clara cells. Taken together, these data suggest that the nonhematopoietic side population isolated from lung is enriched for previously identified airway stem cells. stem cell; Clara; lung; Hoechst 33342 Address for reprint requests and other correspondence: B. R. Stripp, Univ. of Pittsburgh, FORBL Rm. 314, 3343 Forbes Ave., Pittsburgh, PA 15260 (E-mail: brs2{at}pitt.edu ).
ISSN:1040-0605
1522-1504
DOI:10.1152/ajplung.00149.2003