Cross-linked small polyethylenimines: while still nontoxic, deliver DNA efficiently to mammalian cells in vitro and in vivo

Polyethylenimine (PEI) is among the most efficient nonviral gene delivery vectors. Its efficiency and cytotoxicity depend on molecular weight, with the 25-kDa PEI being most efficient but cytotoxic. Smaller PEIs are noncytotoxic but less efficient. Enhancement in gene delivery efficiency with minima...

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Bibliographic Details
Published in:Pharmaceutical research 2005-03, Vol.22 (3), p.373-380
Main Authors: Thomas, Mini, Ge, Qing, Lu, James J, Chen, Jianzhu, Klibanov, Alexander M
Format: Article
Language:English
Subjects:
Animals
Biodegradability
Cell death
Cell Survival - drug effects
Cell Survival - physiology
Cell viability
Chlorocebus aethiops
COS Cells
Cross-Linking Reagents - administration & dosage
Cytotoxicity
DNA - administration & dosage
DNA - genetics
DNA damage
Drug Delivery Systems - methods
Efficiency
Gene transfer
Genetic Therapy - methods
Light scattering
Male
Mammalian cells
Mice
Mice, Inbred C57BL
Molecular weight
Polycations
Polyethyleneimine
Polyethyleneimine - administration & dosage
Size distribution
Trachea
Transfection
β-Galactosidase
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