The C-Terminal Lysines Fine-Tune P53 Stress Responses in a Mouse Model but Are Not Required for Stability Control or Transactivation

• Published in: Proceedings of the National Academy of Sciences - PNAS 2005-07, Vol.102 (29), p.10188-10193
• Main Authors: Krummel, Kurt A., Lee, Crystal J., Toledo, Franck, Wahl, Geoffrey M., Verma, Inder M.
• Format: Article
• Language: English
• Subjects: Alleles; Amino Acid Sequence; Animals; Apoptosis; Apoptosis - genetics; Biochemistry; Biological Sciences; Blotting, Western; Cell cycle; Cell Cycle - genetics; Cellular biology; DNA Damage; DNA Primers; Exons; Flow Cytometry; Fluorescent Antibody Technique; Gene Expression Regulation - genetics; Genetic mutation; Genotype; Genotypes; Irradiation; Life Sciences; Lysine - genetics; Mice; Mice, Mutant Strains; Molecular Sequence Data; Mutation; Mutation - genetics; Proteins; Regulatory Sequences, Nucleic Acid - genetics; Thymus Gland - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; Tumors; Ubiquitins
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0503068102

P53 is an unstable transcription factor that is mutated in a majority of human cancers. With a significant role in initiating cell elimination programs, a network has evolved to fine-tune P53 transcriptional output and prevent errant activation. Modifications of the C terminus have long been viewed as critical binary determinants of P53 stability or activation. However, these conclusions are based on in vitro transfection or biochemical analyses where the stoichiometries between P53 and its regulators are perturbed. Therefore, we tested the importance of the C-terminal regulatory region for P53 control in mice where the seven C-terminal lysines were changed to arginine (Trp-537 KR). Surprisingly, the homozygous mutant mice are viable and phenotypically normal. We have functionally characterized the mutant protein in both MEFs and thymocytes, revealing the unexpected result that Trp-537 KRexhibits a normal half-life and functions like WT P53 in cell cycle arrest and apoptosis, and in an E1A-ras xenograft tumor suppression assay. However, a significant difference is that P537 KRis activated more easily by DNA damage in thymus than WT P53. Importantly, although MEFs encoding WT P53 spontaneously emerge from crisis to become immortal in a 3T3 growth protocol, we do not observe any such escape with the P537 KRcells. We propose that the C-terminal modifications believed to be critical for proper P53 regulation are not essential, but contribute to a fine-tuning mechanism of homeostatic control in vivo.