Electrogenic malate uptake and improved growth energetics of the malolactic bacterium Leuconostoc oenos grown on glucose-malate mixtures

Growth of the malolactic bacterium Leuconostoc oenos was improved with respect to both the specific growth rate and the biomass yield during the fermentation of glucose-malate mixtures as compared with those in media lacking malate. Such a finding indicates that the malolactic reaction contributed t...

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Bibliographic Details
Published in:Journal of Bacteriology 1992-08, Vol.174 (16), p.5302-5308
Main Authors: Loubiere, P. (Centre National de la Recherche Scientifique Unite de Recherche Associee, Toulouse, France), Salou, P, Leroy, M.J, Lindley, N.D, Pareilleux, A
Format: Article
Language:English
Subjects:
ACIDE MALIQUE
ACIDO MALICO
Adenosine Triphosphate - metabolism
Bacteriology
Biological and medical sciences
Biological Transport
Biotechnology
CRECIMIENTO
CROISSANCE
Culture Media
Energy Metabolism
FERMENTACION
FERMENTATION
Fundamental and applied biological sciences. Psychology
GLUCOSA
GLUCOSE
Hydrogen-Ion Concentration
Kinetics
LEUCONOSTOC
Leuconostoc - growth & development
Leuconostoc - metabolism
Leuconostoc oenos
Life Sciences
Malates - metabolism
Metabolism. Enzymes
METABOLISME ENERGETIQUE
METABOLISMO ENERGETICO
Microbiology
Microbiology and Parasitology
Phosphorylation
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Summary:Growth of the malolactic bacterium Leuconostoc oenos was improved with respect to both the specific growth rate and the biomass yield during the fermentation of glucose-malate mixtures as compared with those in media lacking malate. Such a finding indicates that the malolactic reaction contributed to the energy budget of the bacterium, suggesting that growth is energy limited in the absence of malate. An energetic yield (Y(ATP)) of 9.5 g of biomass.mol ATP-1 was found during growth on glucose with an ATP production by substrate-level phosphorylation of 1.2 mol of ATP.mol of glucose-1. During the period of mixed-substrate catabolism, an apparent Y(ATP) of 17.7 was observed, indicating a mixotrophy-associated ATP production of 2.2 mol of ATP.mol of glucose-1, or more correctly an energy gain of 0.28 mol of ATP.mol of malate-1, representing proton translocation flux from the cytoplasm to the exterior of 0.56 or 0.84 H+.mol of malate-1 (depending on the H+/ATP stoichiometry). The growth-stimulating effect of malate was attributed to chemiosmotic transport mechanisms rather than proton consumption by the malolactic enzyme. Lactate efflux was by electroneutral lactate-/H+ symport having a constant stoichiometry, while malate uptake was predominantly by a malate-/H+ symport, though a low-affinity malate- uniport was also implicated. The measured electrical component of the proton motive force was altered, passing from -30 to -60 mV because of this translocation of dissociated organic acids when malolactic fermentation occurred
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.174.16.5302-5308.1992