Analysis of genetic diversity among Staphylococcus aureus isolates from patients with deep-seated and superficial staphylococcal infections using pulsed-field gel electrophoresis

Molecular typing was performed to reveal the genetic diversity among Staphylococcus aureus strains causing deep-seated versus superficial staphylococcal infections. Pulsed-field gel electrophoresis with cluster analysis, plasmid and antimicrobial susceptibility profiling of 50 S. aureus strains coll...

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Bibliographic Details
Published in:Scandinavian journal of infectious diseases 2006, Vol.38 (6-7), p.418-426
Main Authors: Kumar, Ashok, Ray, Pallab, Kanwar, Mamta, Sharma, Meera, Varma, Subhash
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Child
Child, Preschool
Drug Resistance, Bacterial - genetics
Electrophoresis, Gel, Pulsed-Field
Female
Genetic Variation
Humans
Infant
Male
Middle Aged
Staphylococcal Infections - microbiology
Staphylococcal Infections - pathology
Staphylococcus aureus
Staphylococcus aureus - genetics
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Summary:Molecular typing was performed to reveal the genetic diversity among Staphylococcus aureus strains causing deep-seated versus superficial staphylococcal infections. Pulsed-field gel electrophoresis with cluster analysis, plasmid and antimicrobial susceptibility profiling of 50 S. aureus strains collected from these 2 groups of patients were undertaken. A total of 19 (designated A through S) distinct genotypes were identified by PFGE of Sma I-digested genomic DNA. The most prevalent PFGE type was L, which accounted for 30% of isolates and was detected among superficial isolates only. The second most prevalent PFGE type, type A (18%), was predominant among deep-seated isolates. Remaining PFGE types varied in distribution between the 2 groups. Plasmid profile analysis revealed that deep isolates harbour plasmids more frequently (comprising 64% of isolates) than superficial isolates (4%) and showed 10 and 2 distinct patterns, respectively, with pattern 1 being the dominant among deep isolates. Antimicrobial susceptibility data suggested an increased prevalence of antibiotic resistance among deep isolates with the majority (40%) exhibiting identical antibiograms compared to superficial isolates. No resistance was detected against clindamycin and vancomycin. The results of our study indicate a previously unrecognized dichotomy of S. aureus strains, causing deep-seated and superficial infections.
ISSN:0036-5548
1651-1980
DOI:10.1080/00365540500440361