Two Domains of Cytotoxic Necrotizing Factor Type 1 Bind the Cellular Receptor, Laminin Receptor Precursor Protein

• Published in: Infection and Immunity 2007-11, Vol.75 (11), p.5095-5104
• Main Authors: McNichol, Beth A, Rasmussen, Susan B, Carvalho, Humberto M, Meysick, Karen C, O'Brien, Alison D
• Format: Article
• Language: English
• Subjects: Antibodies, Monoclonal - metabolism; Bacterial Toxins - chemistry; Bacterial Toxins - genetics; Bacterial Toxins - metabolism; Bacterial Toxins - toxicity; Bacteriology; Biological and medical sciences; cdc42 GTP-Binding Protein - metabolism; Cell Line, Tumor; Cellular Microbiology: Pathogen-Host Cell Molecular Interactions; Epithelial Cells - chemistry; Escherichia coli; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Escherichia coli Proteins - toxicity; Fundamental and applied biological sciences. Psychology; Humans; Microbiology; Microscopy, Confocal; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Protein Binding; Protein Conformation; Protein Interaction Mapping; Protein Precursors - metabolism; Protein Structure, Tertiary; rac1 GTP-Binding Protein - metabolism; Receptors, Laminin - metabolism; rhoA GTP-Binding Protein - metabolism; Sequence Deletion
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00075-07

Cytotoxic necrotizing factor type 1 (CNF1) and CNF2 are highly homologous toxins that are produced by certain pathogenic strains of Escherichia coli. These 1,014-amino-acid toxins catalyze the deamidation of a specific glutamine residue in RhoA, Rac1, and Cdc42 and consist of a putative N-terminal binding domain, a transmembrane region, and a C-terminal catalytic domain. To define the regions of CNF1 that are responsible for binding of the toxin to its cellular receptor, the laminin receptor precursor protein (LRP), a series of CNF1 truncated toxins were characterized and assessed for toxin binding. In particular, three truncated toxins, ΔN63, ΔN545, and ΔC469, retained conformational integrity and in vitro enzymatic activity and were immunologically reactive against a panel of anti-CNF1 monoclonal antibodies (MAbs). Based on a comparison of these truncated toxins with wild-type CNF1 and CNF2 in LRP and HEp-2 cell binding assays and in MAb and LRP competitive binding inhibition assays and based on the results of confocal microscopy, we concluded that CNF1 contains two major binding regions: one located within the N terminus, which contained amino acids 135 to 164, and one which resided in the C terminus and included amino acids 683 to 730. The data further indicate that CNF1 can bind to an additional receptor(s) on HEp-2 cells and that LRP can also serve as a cellular receptor for CNF2.