A small animal holding fixture system with positional reproducibility for longitudinal multimodal imaging

This study presents a combined small animal holding fixture system, termed a 'bridge capsule', which provides for small animal re-fixation with positional reproducibility. This system comprises separate holding fixtures for the head and lower body and a connecting part to a gas anesthesia...

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Bibliographic Details
Published in:Physics in medicine & biology 2010-07, Vol.55 (14), p.4119-4130
Main Authors: Kokuryo, Daisuke, Kimura, Yuichi, Obata, Takayuki, Yamaya, Taiga, Kawamura, Kazunori, Zhang, Ming-Rong, Kanno, Iwao, Aoki, Ichio
Format: Article
Language:English
Subjects:
Anesthesiology - instrumentation
Animals
Equipment Design
Female
Head - diagnostic imaging
Hindlimb - diagnostic imaging
Ilium - diagnostic imaging
Longitudinal Studies
Magnetic Resonance Imaging - instrumentation
Mice
Mice, Inbred BALB C
Mice, Nude
Motion
Patient Positioning - instrumentation
Positron-Emission Tomography - instrumentation
Radiography
Reproducibility of Results
Software
Spine - diagnostic imaging
Tail - diagnostic imaging
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Summary:This study presents a combined small animal holding fixture system, termed a 'bridge capsule', which provides for small animal re-fixation with positional reproducibility. This system comprises separate holding fixtures for the head and lower body and a connecting part to a gas anesthesia system. A mouse is fixed in place by the combination of a head fixture with a movable part made from polyacetal resin, a lower body fixture made from vinyl-silicone and a holder for the legs and tail. For re-fixation, a similar posture could be maintained by the same holding fixtures and a constant distance between the head and lower body fixtures is maintained. Artifacts caused by the bridge capsule system were not observed on magnetic resonance (MRI) and positron emission tomography (PET) images. The average position differences of the spinal column and the iliac body before and after re-fixation for the same modality were approximately 1.1 mm. The difference between the MRI and PET images was approximately 1.8 mm for the lower body fixture after image registration using fiducial markers. This system would be useful for longitudinal, repeated and multimodal imaging experiments requiring similar animal postures.
ISSN:0031-9155
1361-6560
DOI:10.1088/0031-9155/55/14/011