Characterized Polysaccharides from Black Soybean Induce Granulocyte Colony-Stimulated Factor Gene Expression in a Phosphoinositide 3-Kinase-dependent Manner

• Published in: Immunological investigations 2011, Vol.40 (1), p.39-61
• Main Authors: Wu, Ming-Hsi, Lee, Yi-Ching, Tsai, Wei-Jern, Yang, Wen-Bin, Chen, Yi-Chun, Chuang, Kai-An, Liao, Jyh-Fei, Wang, Chia-Chuan, Kuo, Yuh-Chi
• Format: Article
• Language: English
• Subjects: Adult; Black soybean; Blotting, Western; Butadienes - pharmacology; Chromones - pharmacology; Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors; Extracellular Signal-Regulated MAP Kinases - metabolism; G-CSF; Gene Expression; Glycine max; Granulocyte Colony-Stimulating Factor - biosynthesis; Granulocyte Colony-Stimulating Factor - genetics; Granulocyte Colony-Stimulating Factor - metabolism; Humans; Leukocytes, Mononuclear - metabolism; Male; Morpholines - pharmacology; NF-kappa B - antagonists & inhibitors; NF-kappa B - metabolism; NFATC Transcription Factors - antagonists & inhibitors; NFATC Transcription Factors - metabolism; Nitriles - pharmacology; PBMC; PGM; Phosphatidylinositol 3-Kinases - metabolism; Phosphoinositide-3 Kinase Inhibitors; Phosphorylation - drug effects; PI3K; PKC θ; Polysaccharides - immunology; Protein Kinase C - antagonists & inhibitors; Protein Kinase C - metabolism; Pyrrolidines - pharmacology; RNA, Messenger - genetics; RNA, Messenger - metabolism; Thiocarbamates - pharmacology
• ISSN: 0882-0139; 1532-4311
• DOI: 10.3109/08820139.2010.513021

Black soybean (Glycine max L. merr.) is an edible Chinese medicine for nourishment spleen. In the present study, effects of characterized polysaccharides from black soybean (PGM) on granulocyte colony-stimulated factor (G-CSF) production in human peripheral blood mononuclear cells (PBMC) were determined and their action mechanisms were examined. The results indicated that PGM concentration-dependently enhanced G-CSF production in PBMC through modulation of mRNA expression. Data from Western blotting showed that PGM significantly induced the extracellular signal-regulated protein kinase (ERK) activation in PBMC. The nuclear factor (NF)-κB activation in PBMC was increased with PGM by modulation of IκB degradation and PKC θ activation. The levels of G-CSF mRNA in PGM-treated PBMC could be reduced by ERK inhibitor U0126 and NF-κB inhibitor pyrrolidine dithiocarbamate, respectively. Furthermore, the data showed that PGM stimulated phosphoinositide 3-kinase (PI3K)-regulated Akt phosphorylation. The PI3K inhibitor, Ly294002, blocked ERK, NF-κB, and PKC θ activation and G-CSF mRNA expression in PBMC induced by PGM. Thus, we first proved that the enhancement mechanisms of PGM on G-CSF production, appeared to be mediated, at least in part, through activation of PI3K, ERK, PKC θ, and NF-κB signaling pathways in PBMC. We suggest that PGM from black soybean is a potential G-CSF stimulator.