Cytoplasmic expression of the JM403 antigen GlcA-GlcNH3+ on heparan sulfate glycosaminoglycan in mammary carcinomas—a novel proliferative biomarker for breast cancers with high malignancy

The expressions of heparan sulfate glycosaminoglycans (HSGAGs) in breast carcinoma specimens from 60 patients were immunohistochemically investigated using monoclonal antibodies (mAbs) that recognized different epitopes of the glycan structure. Cytoplasmic expression of GlcA-GlcNH 3 + on HSGAG was d...

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Bibliographic Details
Published in:Glycoconjugate journal 2010-10, Vol.27 (7-9), p.661-672
Main Authors: Fujii, Masahiro, Yusa, Akiko, Yokoyama, Yukihiro, Kokuryo, Toshio, Tsunoda, Nobuyuki, Oda, Koji, Nagino, Masato, Ishimaru, Tsuyoshi, Shimoyama, Yoshie, Utsunomiya, Hirotoshi, Iwata, Hiroji, Itoh, Yoshiko, Itoh, Johbu, Kannagi, Reiji, Kyogashima, Mamoru
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Antigens, Neoplasm - biosynthesis
Biochemistry
Biomarkers, Tumor - biosynthesis
Biomedical and Life Sciences
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Breast Neoplasms - ultrastructure
Cell Proliferation
Cytoplasm - metabolism
Female
Heparan Sulfate Proteoglycans - biosynthesis
Humans
Intestine, Small - cytology
Intestine, Small - metabolism
Ki-67 Antigen - biosynthesis
Life Sciences
Mammary Glands, Human - metabolism
Middle Aged
Pathology
Receptor, ErbB-2 - biosynthesis
Receptors, Estrogen - biosynthesis
Receptors, Progesterone - biosynthesis
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Summary:The expressions of heparan sulfate glycosaminoglycans (HSGAGs) in breast carcinoma specimens from 60 patients were immunohistochemically investigated using monoclonal antibodies (mAbs) that recognized different epitopes of the glycan structure. Cytoplasmic expression of GlcA-GlcNH 3 + on HSGAG was detected in carcinomas at high frequency (58.3%) using mAb JM403, whereas it was almost undetectable in normal breast ducts. This cytoplasmic expression was confirmed using confocal laser scanning microscopy. The expression of JM403 antigen in invasive carcinomas significantly correlated with nuclear atypia score ( p  = 0.0004), mitotic counts score ( p  = 0.0018), nuclear grade ( p  = 0.0061) and the incidence of metastasis to axillary lymph nodes ( p  = 0.0061). Furthermore, its expression was significantly correlated with the Ki67-labeling index in 55 invasive carcinomas ( p  
ISSN:0282-0080
1573-4986
DOI:10.1007/s10719-010-9311-4