Molecular imaging techniques to study the biodistribution of orally administered (99m)Tc-labelled naive and ligand-tagged nanoparticles

Study by molecular imaging the biodistribution of poly(anhydride) nanoparticles after oral administration. Poly (anhydride) nanoparticles (NP) and cyclodextrin-tagged nanoparticles (CD-NP) were radiolabelled with (99m)Tc. Radiochemical purity was measured with a double-solvent chromatography system...

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Bibliographic Details
Published in:Molecular imaging and biology 2011-12, Vol.13 (6), p.1215
Main Authors: Areses, Paloma, Agüeros, Ma Teresa, Quincoces, Gemma, Collantes, María, Richter, José Ángel, López-Sánchez, Luisa Ma, Sánchez-Martínez, María, Irache, Juan M, Peñuelas, Iván
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Cyclodextrins - chemistry
Freeze Drying
Gastric Emptying
Ligands
Male
Molecular Imaging - methods
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Quality Control
Rats
Rats, Wistar
Spectrum Analysis
Technetium - administration & dosage
Technetium - pharmacokinetics
Tissue Distribution
Tomography, Emission-Computed, Single-Photon
Tomography, X-Ray Computed
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Summary:Study by molecular imaging the biodistribution of poly(anhydride) nanoparticles after oral administration. Poly (anhydride) nanoparticles (NP) and cyclodextrin-tagged nanoparticles (CD-NP) were radiolabelled with (99m)Tc. Radiochemical purity was measured with a double-solvent chromatography system and the absence of undesirable components was confirmed by size and polydispersion measurement of the technetium-labelled nanoparticles by photon correlation spectroscopy. Single photon emission computed tomography (SPECT) fused computed tomography (CT) in vivo molecular imaging was used for biodistribution studies in small animals. SPECT-CT images revealed activity only in the gastrointestinal tract. Thirteen percent of the given dose of CD-NP and 3% of the given dose of conventional NP were found in the stomach at 8 h. No evidence of translocation or distribution out of gastrointestinal tract was found. CD-NP moved significantly more slowly inside the gut than conventional NP, probably due to their physico-chemical structure that allows stronger interactions with the gut mucosa.
ISSN:1860-2002
DOI:10.1007/s11307-010-0456-0