Validation of the Flush Method as an Alternative to Basal or Reflex Tear Collection

Purpose: To validate the more easily applicable "flush" tear collection technique as a viable alternative to basal and reflex tear collection. Materials and Methods: Total protein content (TPC) and immunoglobulin A (IgA) concentrations were determined in the basal, reflex, and flush tears...

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Bibliographic Details
Published in:Current eye research 2011-03, Vol.36 (3), p.198-207
Main Authors: Markoulli, Maria, Papas, Eric, Petznick, Andrea, Holden, Brien
Format: Article
Language:English
Subjects:
Adolescent
Adult
Basal tears
Blinking - physiology
Electrophoresis, Polyacrylamide Gel
Eye Proteins - analysis
Female
Flush tears
Human tear film
Humans
Immunoglobulin A, Secretory - analysis
Male
Mass Spectrometry
Middle Aged
Ophthalmology - methods
Reflex tears
Specimen Handling - methods
Tear collection
Tear proteins
Tears - chemistry
Young Adult
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Summary:Purpose: To validate the more easily applicable "flush" tear collection technique as a viable alternative to basal and reflex tear collection. Materials and Methods: Total protein content (TPC) and immunoglobulin A (IgA) concentrations were determined in the basal, reflex, and flush tears of 16 healthy non-contact lens wearers. The overall protein profile was established using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry (MS). Results: Collection-rates were 4.6 ± 6.7 μl/min, 13.9 ± 11.1 μl/min, and 25.7 ± 12.4 μl/min for the basal, reflex, and flush tears, respectively. TPC was 7.14 ± 2.22 mg/mL, 6.01 ± 2.11 mg/mL, and 3.79 ± 1.51mg/ mL for basal, reflex, and flush tears, respectively, with flush tears being significantly less concentrated than basal (p = 0.001) and reflex (p = 0.008). IgA concentration was 1.04 ± 0.29 mg/ mL, 0.64 ± 0.26 mg/mL, and 0.65 ± 0.23 mg/mL for basal, reflex, and flush tears, respectively, with basal tears being significantly more concentrated (p 
ISSN:0271-3683
1460-2202
DOI:10.3109/02713683.2010.542867