An Endogenous Lectin and One of Its Neuronal Glycoprotein Ligands are Involved in Contact Guidance of Neuron Migration

In the central nervous system, postmitotic neurons migrate along astrocytic processes to reach their adult position. The molecular mechanisms of this guided migration are not clearly defined, although some steps have been shown to involve proteases and cell adhesion molecules. We report that monoval...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1990-08, Vol.87 (16), p.6455-6459
Main Authors: Lehmann, Sylvain, Kuchler, Sabine, Theveniau, Magali, Vincendon, Guy, Zanetta, Jean-Pierre
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Monoclonal
Astrocytes
Autoradiography
Biological and medical sciences
Cell Adhesion
Cell Communication
Cell differentiation, maturation, development, hematopoiesis
Cell Movement
Cell physiology
Cerebellum
Cerebellum - physiology
CSL protein
Cultured cells
DNA Replication
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Glial Fibrillary Acidic Protein - analysis
Glycoproteins
Glycoproteins - physiology
Lectins
Lectins - physiology
Ligands
Membrane Glycoproteins - physiology
migration
Models, Neurological
Molecular and cellular biology
Molecules
Neurons
Neurons - cytology
Neurons - physiology
Neurons - ultrastructure
Organ Culture Techniques
Polysaccharides
Rats
Thymidine - metabolism
Tritium
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Summary:In the central nervous system, postmitotic neurons migrate along astrocytic processes to reach their adult position. The molecular mechanisms of this guided migration are not clearly defined, although some steps have been shown to involve proteases and cell adhesion molecules. We report that monovalent antibodies (Fab fragments) raised against an endogenous cerebellar soluble lectin (CSL) completely inhibit neuronal migration in cultures of cerebellar explants at concentrations as low as 50 μg/ml. A similar inhibition pattern was obtained with Fab fragments prepared against one of the endogenous glycoprotein ligands of CSL, the 31-kDa glycoprotein (this glycoprotein is a membrane-bound glycoprotein specifically occurring, in the cerebellum, at the surface of immature neurons). We propose that this lectin-glycoprotein interaction supports the adhesion between neurons and the astrocyte guide during the migration of cerebellar immature neurons.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.87.16.6455