Aptamer selection by high-throughput sequencing and informatic analysis

Traditional methods for selecting aptamers require multiple rounds of selection and optimization in order to identify aptamers that bind with high affinity to their targets. Here we describe an assay that requires only one round of positive selection followed by high-throughput DNA sequencing and in...

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Bibliographic Details
Published in:	BioTechniques 2011-12, Vol.51 (6), p.413-416
Main Authors:	Hoon, Shawn, Zhou, Bin, Janda, Kim D, Brenner, Sydney, Scolnick, Jonathan
Format:	Article
Language:	English
Subjects:	Affinity aptamer Aptamers, Nucleotide - genetics Aptamers, Nucleotide - metabolism Assaying Biological and medical sciences Biology Constants Diverse techniques Fundamental and applied biological sciences. Psychology Gene Library Gene sequencing high throughput sequencing High-Throughput Nucleotide Sequencing - economics High-Throughput Nucleotide Sequencing - methods Humans informatics Molecular and cellular biology Nanostructure Optimization Protein Binding SELEX Aptamer Technique - economics SELEX Aptamer Technique - methods Sequencing Thrombin Thrombin - metabolism
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