Protective effect of apocynin in an established alcoholic steatohepatitis rat model

Apocynin is a widely used antioxidant in both basic and clinical research. The current study aimed to investigate the protective effect of apocynin in an established alcoholic steatohepatitis rat model. Healthy SD rats were gastrically fed with ethanol (4.0 g/kg) for 8 weeks to induce alcoholic stea...

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Published in:Immunopharmacology and immunotoxicology 2012-08, Vol.34 (4), p.633-638
Main Authors: Fan, Rengen, Shan, Xiangxiang, Qian, Haixin, Song, Changzhi, Wu, Guangzhou, Chen, Yan, Miao, Yufeng, Cha, Wenzhang
Format: Article
Language:English
Subjects:
Acetophenones - pharmacology
Alcoholic steatohepatitis
Animals
Antioxidants - pharmacology
apocynin
Biomarkers - metabolism
Central Nervous System Depressants - adverse effects
Central Nervous System Depressants - pharmacology
Disease Models, Animal
Ethanol - adverse effects
Ethanol - pharmacology
Fatty Liver, Alcoholic - drug therapy
Fatty Liver, Alcoholic - metabolism
Fatty Liver, Alcoholic - pathology
hepatic injury
Liver - metabolism
Liver - pathology
Male
NADPH oxidase
NADPH Oxidases - metabolism
Oxidative Stress - drug effects
RAS
Rats
Rats, Sprague-Dawley
Renin-Angiotensin System - drug effects
Time Factors
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Summary:Apocynin is a widely used antioxidant in both basic and clinical research. The current study aimed to investigate the protective effect of apocynin in an established alcoholic steatohepatitis rat model. Healthy SD rats were gastrically fed with ethanol (4.0 g/kg) for 8 weeks to induce alcoholic steatohepatitis. After 8 weeks, rats were fed with ethanol for another 2 weeks with or without a daily intraperitoneal injection of 25 mg/kg apocynin. After sacrificing, serum and liver samples were subjected to hepatic injury measurements. After 8-week ethanol induction, rats exhibited typical alcoholic steatohepatitis features including reduced body weight, hepatic histological changes, elevated serum alanine transaminase (ALT) level, increased levels of oxidative stress and inflammatory markers, NADPH oxidases, and renin-angiotensin system (RAS) marker. Co-treatment of apocynin alleviated the hepatic injury and biochemical parameters induced by alcoholic steatohepatitis. In conclusion, addition of apocynin significantly attenuates hepatic oxidative stress and inflammation induced by alcoholic steatohepatitis. This effect is partly through the inhibition of the RAS system.
ISSN:0892-3973
1532-2513
DOI:10.3109/08923973.2011.648266