CIM® monolithic anion-exchange chromatography as a useful alternative to CsCl gradient purification of bacteriophage particles

The use of anion-exchange chromatography was investigated as an alternative method to concentrate and purify bacterial viruses, and parameters for different bacteriophages were compared. Chromatography was performed with Convective Interactive Media® monoliths, with three different volumes and two m...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2012-12, Vol.434 (2), p.265-270
Main Authors: Adriaenssens, Evelien M., Lehman, Susan M., Vandersteegen, Katrien, Vandenheuvel, Dieter, Philippe, Didier L., Cornelissen, Anneleen, Clokie, Martha R.J., García, Andrés J., De Proft, Maurice, Maes, Martine, Lavigne, Rob
Format: Article
Language:English
Subjects:
Anion-exchange chromatography
Bacteriophages
Bacteriophages - isolation & purification
buffers
Centrifugation, Density Gradient - methods
Cesium
Chlorides
Chromatography, Ion Exchange - methods
Infectious Disease
Purification
purification methods
Virology - methods
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