Genotoxicity of structurally related copper and zinc containing Schiff base complexes

• Published in: Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 2013-10, Vol.36 (4), p.435-442
• Main Authors: Khabour, Omar F., Saleh, Na'il, Alzoubi, Karem H., Hisaindee, Soleiman, Al-Fyad, Doaa, Al-Kaabi, Leena, Dodeen, Arwa, Esmadi, Fatima T.
• Format: Article
• Language: English
• Subjects: 8-OH-dG; Animals; chromosomal aberrations; Chromosome Aberrations - chemically induced; copper; Copper - metabolism; Copper - toxicity; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - urine; DNA damage; DNA Damage - drug effects; Dose-Response Relationship, Drug; Lymphocytes - drug effects; Molecular Structure; Mutagenicity Tests - methods; Naphthalenes - chemistry; Rats; Schiff bases; Schiff Bases - chemistry; Schiff Bases - metabolism; Schiff Bases - toxicity; sister chromatid exchange; Sister Chromatid Exchange - drug effects; Spectrophotometry, Ultraviolet; zinc; Zinc - metabolism; Zinc - toxicity
• ISSN: 0148-0545; 1525-6014
• DOI: 10.3109/01480545.2013.776577

Abstract The utilization of Schiff bases in the industrial and pharmaceutical fields has led to an increase in their syntheses and evaluation of their biological activities. In this study, we described the synthesis and genotoxicity of two Schiff bases that share common platform in their construction, namely, naphthalene, and are complexed to either Cu(II) or Zn(II). The genotoxicity of these complexes was evaluated in cultured lymphocytes using sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs), and in rats using the urine 8-OH-2-deoxyguanosine (8-OH-dG) assay. The results showed that the examined complexes are genotoxic, but with different degrees. The order of genotoxicity of the complexes at 10 µg/mL was: Cu(L3)(NCS)(H2O) > Zn(L3)(NCS)(H2O) > Cu(L2)(NCS) > Zn(L2)(NCS), where L2 and L3 are the conjugate bases of N-(8-quinolyl)napthaldimine and N-(anilinyl)napthaldimine, respectively. However, at the 1-µg/mL concentration, only the Cu(L3)(NCS)(H2O) complex induced significant CAs, whereas at the 0.1-µg/mL concentration, only Cu(L3)(NCS)(H2O) and Zn(L2)(NCS) complexes induced significant SCEs, compared to controls. In the urine 8-OH-dG assay, all complexes at 10 mg/100 g body weight (b.w.) were found to cause DNA damage with the following order: Cu(L3)(NCS)(H2O) > Zn(L2)(NCS) > Zn(L3)(NCS)(H2O) > Cu(L2)(NCS), whereas no significant DNA damage was observed in animals exposed to 1 and 0.1 mg/100 g b.w. (p > 0.05). In conclusion, the two examined Schiff base complexes are found to induce DNA damage, but with different degrees.