NADH-dependent glutamate synthase participated in ammonium assimilation in Arabidopsis root

Higher plants have 2 GOGAT species, Fd-GOGAT and NADH-GOGAT. While Fd-GOGAT mainly assimilates ammonium in leaves, which is derived from photorespiration, the function of NADH-GOGAT, which is highly expressed in roots, (1) needs to be elucidated. The aim of this study was to clarify the role of NADH...

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Bibliographic Details
Published in:Plant signaling & behavior 2014, Vol.9 (8), p.e29402
Main Authors: Kojima, Soichi, Konishi, Noriyuki, Beier, Marcel Pascal, Ishiyama, Keiki, Maru, Ikumi, Hayakawa, Toshihiko, Yamaya, Tomoyuki
Format: Article
Language:English
Subjects:
Amino Acid Oxidoreductases - metabolism
Ammonium Compounds - metabolism
Arabidopsis - enzymology
Arabidopsis - growth & development
Arabidopsis - metabolism
Biomass
DNA, Bacterial
Glutamate Synthase (NADH) - metabolism
Glutamic Acid - metabolism
Light
Nitrogen - metabolism
Plant Roots - metabolism
Plants, Genetically Modified
Promoter Regions, Genetic
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Summary:Higher plants have 2 GOGAT species, Fd-GOGAT and NADH-GOGAT. While Fd-GOGAT mainly assimilates ammonium in leaves, which is derived from photorespiration, the function of NADH-GOGAT, which is highly expressed in roots, (1) needs to be elucidated. The aim of this study was to clarify the role of NADH-GOGAT in Arabidopsis roots. The supply of ammonium to the roots caused an accumulation of NADH-GOGAT, while Fd-GOGAT 1 and Fd-GOGAT 2 showed no response. A promoter-GUS fusion analysis and immunohistochemistry showed that NADH-GOGAT was located in non-green tissues like vascular bundles, shoot apical meristem, pollen, stigma, and roots. The localization of NADH-GOGAT and Fd-GOGAT was not overlapped. NADH-GOGAT T-DNA insertion lines showed a reduction of glutamate and biomass under normal CO2 conditions. These data emphasizes the importance of NADH-GOGAT in the ammonium assimilation of Arabidopsis roots.
ISSN:1559-2324
DOI:10.4161/psb.29402