Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer

The development of simple, accurate, rapid and cost-effective technologies for mutation detection is crucial to the early diagnosis and prevention of numerous genetic diseases, pharmacogenetics, and drug resistance. Proofreading PCR (PR-PCR) was developed for mutation detection in 1998 but is rarely...

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Bibliographic Details
Published in:	PloS one 2015-04, Vol.10 (4), p.e0123468
Main Authors:	Hao, Weiming, Fan, Lujuan, Chen, Qianqian, Chen, Xiaoxiang, Zhang, Sichao, Lan, Ke, Lu, Jian, Zhang, Chiyu
Format:	Article
Language:	English
Subjects:	Accuracy Amplification Breast cancer Deoxyribonucleic acid Dideoxynucleotides - chemistry DNA DNA - chemistry DNA - genetics DNA polymerase DNA Primers - chemistry DNA-directed DNA polymerase Drug resistance Editing Genes, p53 - genetics Hospitals Humans Life sciences Methods Mutagenesis, Insertional - genetics Mutation Mycobacterium tuberculosis - genetics Nucleotides p53 Protein Pharmacogenetics Pharmacology Plasmids Point Mutation - genetics Polymerase chain reaction Polymerase Chain Reaction - methods Polymerization Polymorphism Proofreading Rifampin Selectivity Sequence Deletion - genetics Taq Polymerase - chemistry Tuberculosis
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