(1)H NMR based metabolomics approach to study the toxic effects of dichlorvos on goldfish (Carassius auratus)

Dichlorvos (DDVP), one of the most widely used organophosphorus pesticides (OPs), has caused serious pollution in environment. In this study, (1)H nuclear magnetic resonance (NMR) based metabolomics approach combined with histopathological and immunohistochemical examination, and biochemical assays...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2015-11, Vol.138, p.537
Main Authors: Liu, Yan, Chen, Ting, Li, Ming-Hui, Xu, Hua-Dong, Jia, Ai-Qun, Zhang, Jian-Fa, Wang, Jun-Song
Format: Article
Language:English
Subjects:
Animals
Astrocytes - drug effects
Astrocytes - metabolism
Dichlorvos - toxicity
Dose-Response Relationship, Drug
Ecotoxicology
Environmental Pollutants - toxicity
Glutathione - metabolism
Goldfish - metabolism
Humans
Magnetic Resonance Spectroscopy
Metabolomics - methods
Neurotransmitter Agents - metabolism
Organ Specificity
Oxidation-Reduction - drug effects
Oxidative Stress - drug effects
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Summary:Dichlorvos (DDVP), one of the most widely used organophosphorus pesticides (OPs), has caused serious pollution in environment. In this study, (1)H nuclear magnetic resonance (NMR) based metabolomics approach combined with histopathological and immunohistochemical examination, and biochemical assays were used to investigate toxicities of DDVP on goldfish (Carassius auratus). After 10 days' exposure of DDVP at three dosages of 5.18, 2.59 and 1.73 mg/L, goldfish tissues (gill, brain, liver and kidney) and serum were collected. Histopathology revealed severe impairment of gills, livers and kidneys, and immunohistochemistry disclosed glial fibrillary acidic protein (GFAP) positive reactive astrocytes in brains. Orthogonal signal correction-partial least squares-discriminant analysis (OSC-PLS-DA) of NMR profiles disclosed that DDVP influenced many metabolites (glutamate, aspartate, acetylcholine, 4-aminobutyrate, glutathione, AMP and lactate in brain; glutathione, glucose, histamine in liver; BCAAs, AMP, aspartate, glutamate, riboflavin in kidney) dose-dependently, involved with imbalance of neurotransmitters, oxidative stress, and disorders of energy and amino acid metabolism. Several self-protection mechanisms concerning glutamate degradation and glutathione (GSH) redox system were found in DDVP intoxicated goldfish.
ISSN:1879-1298
DOI:10.1016/j.chemosphere.2015.07.030