Eugenia dysenterica DC. (Myrtaceae) exerts chemopreventive effects against hexavalent chromium-induced damage in vitro and in vivo

• Published in: Pharmaceutical biology 2016-11, Vol.54 (11), p.2652-2663
• Main Authors: Ávila, Renato Ivan de, Mattos Alvarenga, Cátia Belo, Ávila, Paulo Henrique Marcelino de, Moreira, Roger Cardoso, Arruda, Andréa Fernandes, Fernandes, Thaís de Oliveira, Rodrigues, Bruna dos Santos, Andrade, Wanessa Machado, Batista, Aline Carvalho, Paula, José Realino de, Valadares, Marize Campos
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - pharmacology; Apoptosis; Caspases - metabolism; Cell Cycle - drug effects; Cells, Cultured; Chromium - toxicity; Eugenia; hepatotoxicity; Kidney - drug effects; Kidney - pathology; Liver - drug effects; Liver - pathology; Male; Membrane Potential, Mitochondrial - drug effects; Mice; nephrotoxicity; oxidative stress; Plant Extracts - analysis; Plant Extracts - pharmacology; Reactive Oxygen Species - metabolism
• ISSN: 1388-0209; 1744-5116
• DOI: 10.1080/13880209.2016.1178306

Context: Eugenia dysenterica DC. (Myrtaceae) has been widely used in the folk medicine and it presents phytochemicals constituents associated to antioxidant properties. Objective: The objective of this study was to investigate the protective effects of E. dysenterica leaf hydroalcoholic extract (EDE) in vitro and in vivo using AMJ2-C11 cells and Swiss mice exposed to hexavalent chromium [Cr(VI)], respectively. Materials and methods: AMJ2-C11 cells were pretreated with EDE and exposed to Cr(VI) to evaluate cytotoxicity and the pathways involved in the chemopreventive effects of the extract. Mice were daily pretreated with EDE and then exposed to Cr(VI). Survival analysis, histopathological examination and determination of Cr levels in biological tissues were carried out. Results: In vitro studies showed that pretreatment of the AMJ2-C11 cells with EDE protected against the cytotoxicity and oxidative stress induced by Cr(VI). Consequently, the pretreatment with EDE reduced reactive oxygen species and apoptosis triggered by Cr(VI), probably by a marked antioxidant and chelating activities demonstrated by EDE. Regarding in vivo studies, pretreatment for 10 days with EDE increased survival of the mice exposed to Cr(VI). In addition, EDE prevented liver and kidney pathological damages, in parallel with reduction in chromium levels found in these organs and plasma. EDE also showed a marked antioxidant potential associated with the presence of polyphenols, especially flavonoids and tannins, as confirmed by HPLC-PDA. Conclusion: The study showed that EDE protects against Cr(VI)-induced damage in vitro and in vivo supporting further studies for the development of therapeutic products applied to prevent the damage induced by toxic metals, especially Cr(VI).