Transcriptional Heterogeneity of Beta Cells in the Intact Pancreas

Pancreatic beta cells have been shown to be heterogeneous at multiple levels. However, spatially interrogating transcriptional heterogeneity in the intact tissue has been challenging. Here, we developed an optimized protocol for single-molecule transcript imaging in the intact pancreas and used it t...

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Bibliographic Details
Published in:	Developmental cell 2019-01, Vol.48 (1), p.115-125.e4
Main Authors:	Farack, Lydia, Golan, Matan, Egozi, Adi, Dezorella, Nili, Bahar Halpern, Keren, Ben-Moshe, Shani, Garzilli, Immacolata, Tóth, Beáta, Roitman, Lior, Krizhanovsky, Valery, Itzkovitz, Shalev
Format:	Article
Language:	English
Subjects:	Animals beta cell heterogeneity diabetes Diabetes Mellitus, Experimental - metabolism Genetic Heterogeneity Glucose - metabolism Insulin - metabolism insulin secretion Insulin Secretion - physiology Insulin-Secreting Cells - metabolism Islets of Langerhans - metabolism Mice, Transgenic mRNA polarization pancreas Pancreas - metabolism Proinsulin - metabolism RNA intracellular localization single molecule Fluorescence in-situ Hybridization
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