Satureja hortensis induces cell death and inhibited cell cycle progression in K562 myelogenous and Jurkat T cell leukemia cell lines

Several plants of Satureja genus have shown anti-tumor activity. We investigated the antileukemia effects of different fractions of Satureja hortensis (Summer savory). The growth inhibitory effect of S. hortensis fractions on K562 and Jurkat leukemia cells were determined by MTT assay. The most effe...

Full description

Saved in:
Bibliographic Details
Published in:Journal of immunoassay & immunochemistry 2019-09, Vol.40 (5), p.459-472
Main Authors: Asadipour, Morvarid, Amirghofran, Zahra
Format: Article
Language:English
Subjects:
Annexin V
Anticancer properties
Antitumor agents
Apoptosis
Biotechnology
Caspase
Caspase-3
Cell cycle
Cell death
Colorimetry
Dichloromethane
Flow cytometry
G1 phase
Leukemia
leukemia cell line
Lymphocytes
Lymphocytes T
medicinal plants
Satureja hortensis
Summer savory
Tumor cell lines
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Several plants of Satureja genus have shown anti-tumor activity. We investigated the antileukemia effects of different fractions of Satureja hortensis (Summer savory). The growth inhibitory effect of S. hortensis fractions on K562 and Jurkat leukemia cells were determined by MTT assay. The most effective fractions were analyzed by flow cytometry and colorimetric assay for apoptosis induction and cell cycle changes. Various fractions from S. hortensis showed growth inhibitory effects on leukemia cells, among them two hexane and dichloromethane fractions with IC 50 values of 32.1-47.8 μg/ml (K562) and 44.3-45.7 μg/ml (Jurkat) were the most effective. According to annexin V staining, both of these fractions significantly induced apoptosis at 50μg/ml in K562 (hexane; 73.06 ± 5.11% and dichloromethane; 96.14 ± 2.33%) and Jurkat cells (hexane; 78.85 ± 11.9% and dichloromethane; 94.05 ± 2.47%) 48 h after treatment. They increased cell accumulation in sub-G1 phase (>50%, p < .001) and decreased number of cells in G0-G1, S and G2M phases. The fractions significantly increased the caspase-3 activity in both cell lines (≈2.5-3.5 fold of untreated cells). Hexane and dichloromethane fractions of S. hortensis had the capacity to induce death and change the cell cycle distribution in leukemia cells; therefore they might be good candidates for more studies in regard to their possible therapeutic usefulness in leukemia.
ISSN:1532-1819
1532-4230
DOI:10.1080/15321819.2019.1629592