Expanding targeting scope, editing window, and base transition capability of base editing in Corynebacterium glutamicum

CRISPR/Cas9‐guided cytidine deaminase enables C:G to T:A base editing in bacterial genome without introduction of lethal double‐stranded DNA break, supplement of foreign DNA template, or dependence on inefficient homologous recombination. However, limited by genome‐targeting scope, editing window, a...

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Bibliographic Details
Published in:	Biotechnology and bioengineering 2019-11, Vol.116 (11), p.3016-3029
Main Authors:	Wang, Yu, Liu, Ye, Li, Junwei, Yang, Yi, Ni, Xiaomeng, Cheng, Haijiao, Huang, Teng, Guo, Yanmei, Ma, Hongwu, Zheng, Ping, Wang, Meng, Sun, Jibin, Ma, Yanhe
Format:	Article
Language:	English
Subjects:	Adenine Adenosine Adenosine deaminase Amino acid substitution Amino acids Bacteria base editing Corynebacterium glutamicum CRISPR Cytidine deaminase Deactivation Deoxyribonucleic acid Dependence Deregulation DNA DNA damage Editing Feedback inhibition Gene sequencing Genomes gRNA design Homologous recombination Homology Inactivation Lysine Metabolic engineering Metabolism nucleobase deaminase PAM requirement
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