Resistance training upregulates skeletal muscle Na + , K + -ATPase content, with elevations in both α 1 and α 2 , but not β isoforms

• Published in: European journal of applied physiology 2020-08, Vol.120 (8), p.1777
• Main Authors: Altarawneh, Muath M, Petersen, Aaron C, Farr, Trevor, Garnham, Andrew, Broatch, James R, Halson, Shona, Bishop, David J, McKenna, Michael J
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological; Adult; Humans; Male; Muscle, Skeletal - metabolism; Muscle, Skeletal - physiology; Protein Isoforms - genetics; Protein Isoforms - metabolism; Resistance Training; Sodium-Potassium-Exchanging ATPase - genetics; Sodium-Potassium-Exchanging ATPase - metabolism; Up-Regulation
• ISSN: 1439-6327
• DOI: 10.1007/s00421-020-04408-3

The Na , K -ATPase (NKA) is important in regulating trans-membrane ion gradients, cellular excitability and muscle function. We investigated the effects of resistance training in healthy young adults on the adaptability of NKA content and of the specific α and β isoforms in human skeletal muscle. Twenty-one healthy young males (22.9 ± 4.6 year; 1.80 ± 0.70 m, 85.1 ± 17.8 kg, mean ± SD) underwent 7 weeks of resistance training, training three times per week (RT, n = 16) or control (CON, n = 5). The training program was effective with a 39% gain in leg press muscle strength (p = 0.001). A resting vastus lateralis muscle biopsy was taken before and following RT or CON and assayed for NKA content ([ H]ouabain binding site content) and NKA isoform (α , α , β β ) abundances. After RT, each of NKA content (12%, 311 ± 76 vs 349 ± 76 pmol g wet weight , p = 0.01), NKA α (32%, p = 0.01) and α (10%, p