m 5 C modification of mRNA serves a DNA damage code to promote homologous recombination

Recruitment of DNA repair proteins to DNA damage sites is a critical step for DNA repair. Post-translational modifications of proteins at DNA damage sites serve as DNA damage codes to recruit specific DNA repair factors. Here, we show that mRNA is locally modified by m C at sites of DNA damage. The...

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Bibliographic Details
Published in:Nature communications 2020-06, Vol.11 (1), p.2834
Main Authors: Chen, Hao, Yang, Haibo, Zhu, Xiaolan, Yadav, Tribhuwan, Ouyang, Jian, Truesdell, Samuel S, Tan, Jun, Wang, Yumin, Duan, Meihan, Wei, Leizhen, Zou, Lee, Levine, Arthur S, Vasudevan, Shobha, Lan, Li
Format: Article
Language:English
Subjects:
Animals
Cell Line, Tumor
Cytosine - metabolism
DNA (Cytosine-5-)-Methyltransferases - genetics
DNA (Cytosine-5-)-Methyltransferases - metabolism
DNA Breaks, Double-Stranded
Drug Resistance, Neoplasm - genetics
Gene Knockdown Techniques
Homologous Recombination
Humans
Methylation
Mice
Neoplasms - drug therapy
Neoplasms - genetics
Neoplasms - pathology
Poly(ADP-ribose) Polymerase Inhibitors - pharmacology
Poly(ADP-ribose) Polymerase Inhibitors - therapeutic use
Rad51 Recombinase - metabolism
Rad52 DNA Repair and Recombination Protein - metabolism
RNA Processing, Post-Transcriptional - genetics
RNA, Messenger - metabolism
RNA, Small Interfering - metabolism
Xenograft Model Antitumor Assays
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Summary:Recruitment of DNA repair proteins to DNA damage sites is a critical step for DNA repair. Post-translational modifications of proteins at DNA damage sites serve as DNA damage codes to recruit specific DNA repair factors. Here, we show that mRNA is locally modified by m C at sites of DNA damage. The RNA methyltransferase TRDMT1 is recruited to DNA damage sites to promote m C induction. Loss of TRDMT1 compromises homologous recombination (HR) and increases cellular sensitivity to DNA double-strand breaks (DSBs). In the absence of TRDMT1, RAD51 and RAD52 fail to localize to sites of reactive oxygen species (ROS)-induced DNA damage. In vitro, RAD52 displays an increased affinity for DNA:RNA hybrids containing m C-modified RNA. Loss of TRDMT1 in cancer cells confers sensitivity to PARP inhibitors in vitro and in vivo. These results reveal an unexpected TRDMT1-m C axis that promotes HR, suggesting that post-transcriptional modifications of RNA can also serve as DNA damage codes to regulate DNA repair.
ISSN:2041-1723