OpenCell: Endogenous tagging for the cartography of human cellular organization

Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven de...

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Bibliographic Details
Published in:	Science (American Association for the Advancement of Science) 2022-03, Vol.375 (6585), p.eabi6983
Main Authors:	Cho, Nathan H., Cheveralls, Keith C., Brunner, Andreas-David, Kim, Kibeom, Michaelis, André C., Raghavan, Preethi, Kobayashi, Hirofumi, Savy, Laura, Li, Jason Y., Canaj, Hera, Kim, James Y. S., Stewart, Edna M., Gnann, Christian, McCarthy, Frank, Cabrera, Joana P., Brunetti, Rachel M., Chhun, Bryant B., Dingle, Greg, Hein, Marco Y., Huang, Bo, Mehta, Shalin B., Weissman, Jonathan S., Gómez-Sjöberg, Rafael, Itzhak, Daniel N., Royer, Loïc A., Mann, Matthias, Leonetti, Manuel D.
Format:	Article
Language:	English
Subjects:	Artificial Intelligence Cartography Cell lines Cellular communication Cluster Analysis Clustering CRISPR CRISPR-Cas Systems Cytology Datasets Datasets as Topic Filaments Fluorescent Dyes Fluoroscopic imaging Gene expression Gene mapping Genome editing Genomes HEK293 Cells High-throughput screening Humans Image analysis Image processing Immunoprecipitation Individualized Instruction Learning algorithms Libraries Localization Machine Learning Marking Mass Spectrometry Mass spectroscopy Microscopy Microscopy, Confocal Nucleotide sequence Organic Chemistry Peptide mapping Protein interaction Protein Interaction Mapping Proteins Proteins - metabolism Proteome - metabolism Proteomes Proteomics - methods Ribonucleic acid RNA RNA-binding protein RNA-Binding Proteins - metabolism Scientific imaging Signatures Spatial Analysis Spatial distribution Spectroscopy Subgroups Tags Websites
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