Semi-nested RT-PCR enables sensitive and high-throughput detection of SARS-CoV-2 based on melting analysis

[Display omitted] •A semi-nested, heptaplex RT-PCR assay for SARS-CoV-2 detection has been developed.•The complex melting spectrum was interpreted by an artificial intelligence algorithm.•The developed assay enables 96-sample pooled testing for increase of testing capacity.•About 8,000 pre-amplified...

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Published in:	CLINICA CHIMICA ACTA 2022-06, Vol.531, p.309-317
Main Authors:	Thi Nguyen, Ngoc Anh, Thi Bui, Hoai, Thi-Huong Pham, Quynh, Thi Thao Hoang, Ly, Xuan Ta, Hung, Heikkinen, Timo, Van Le, Duyet, Dinh Van, Trang, Quoc Ngo, Nam, Thi Hong Huynh, Phuong, Thi Huyen Tran, Trang, Quoc Phan, Hoan, Van Hoang, Luong, van Doorn, H.Rogier, Thi Ngoc Nguyen, Diep, Thi Nguyen, Tam, Sy Vo, Nam, Viet Vo, Cuong, Khac Trinh, Sau, The Pham, Tai, Duc Le, Quang, Van Le, Phan, Thai Nguyen, Son, Thi Tran, Loan, Dinh Vu, Toan, Vu Nguyen, Quynh Anh, Thi Trieu, Nguyet, Thi Le, Thuy, Dinh Nguyen, Ung, Steman, Jakob, Huu Ho, Tho
Format:	Article
Language:	English
Subjects:	Artificial Intelligence Artificial intelligent COVID-19 COVID-19 - diagnosis High-throughput PCR Humans Melting analysis Pandemics Pooling Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics SARS-CoV-2 - genetics Semi-nested Sensitivity and Specificity
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