Real-time denoising enables high-sensitivity fluorescence time-lapse imaging beyond the shot-noise limit

A fundamental challenge in fluorescence microscopy is the photon shot noise arising from the inevitable stochasticity of photon detection. Noise increases measurement uncertainty and limits imaging resolution, speed and sensitivity. To achieve high-sensitivity fluorescence imaging beyond the shot-no...

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Bibliographic Details
Published in:Nature biotechnology 2023-02, Vol.41 (2), p.282-292
Main Authors: Li, Xinyang, Li, Yixin, Zhou, Yiliang, Wu, Jiamin, Zhao, Zhifeng, Fan, Jiaqi, Deng, Fei, Wu, Zhaofa, Xiao, Guihua, He, Jing, Zhang, Yuanlong, Zhang, Guoxun, Hu, Xiaowan, Chen, Xingye, Zhang, Yi, Qiao, Hui, Xie, Hao, Li, Yulong, Wang, Haoqian, Fang, Lu, Dai, Qionghai
Format: Article
Language:English
Subjects:
631/114/1564
631/1647/245/2225
631/1647/328
631/1647/794
Agriculture
Animals
Bioinformatics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Brain injury
Brain research
Calcium imaging
Data processing
Deep learning
Experiments
Fluorescence
Fluorescence microscopy
Head injuries
Image resolution
Interrogation
Laboratories
Leukocyte migration
Leukocytes (neutrophilic)
Life Sciences
Memory
Mice
Microscopy
Microscopy, Fluorescence
Neural networks
Neuroimaging
Noise measurement
Noise reduction
Optimization
Photons
Real time
Sensitivity
Shot noise
Signal processing
Signal to noise ratio
Stochasticity
Time-Lapse Imaging
Traumatic brain injury
Zebrafish
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