A heated rock crack captures and polymerizes primordial DNA and RNA

• Published in: Physical chemistry chemical physics : PCCP 2023-01, Vol.25 (4), p.3375-3386
• Main Authors: Dirscherl, Christina F, Ianeselli, Alan, Tetiker, Damla, Matreux, Thomas, Queener, Robbin M, Mast, Christof B, Braun, Dieter
• Format: Article
• Language: English
• Subjects: Copolymerization; Deoxyribonucleic acid; Diffusion barriers; Dilution; DNA; Equilibrium; Finite element method; Heat flux; Heterogeneity; Hot Temperature; Nucleotides; Polymerization; Polymers; Ribonucleic acid; RNA
• ISSN: 1463-9076; 1463-9084
• DOI: 10.1039/d2cp04538a

Life is based on informational polymers such as DNA or RNA. For their polymerization, high concentrations of complex monomer building blocks are required. Therefore, the dilution by diffusion poses a major problem before early life could establish a non-equilibrium of compartmentalization. Here, we explored a natural non-equilibrium habitat to polymerize RNA and DNA. A heat flux across thin rock cracks is shown to accumulate and maintain nucleotides. This boosts the polymerization to RNA and DNA inside the crack. Moreover, the polymers remain localized, aiding both the creation of longer polymers and fostering downstream evolutionary steps. In a closed system, we found single nucleotides concentrate 10 4 -fold at the bottom of the crack compared to the top after 24 hours. We detected enhanced polymerization for 2 different activation chemistries: aminoimidazole-activated DNA nucleotides and 2′,3′-cyclic RNA nucleotides. The copolymerization of 2′,3′-cGMP and 2′,3′-cCMP in the thermal pore showed an increased heterogeneity in sequence composition compared to isothermal drying. Finite element models unravelled the combined polymerization and accumulation kinetics and indicated that the escape of the nucleotides from such a crack is negligible over a time span of years. The thermal non-equilibrium habitat establishes a cell-like compartment that actively accumulates nucleotides for polymerization and traps the resulting oligomers. We argue that the setting creates a pre-cellular non-equilibrium steady state for the first steps of molecular evolution. Life is based on informational polymers such as DNA or RNA. For their polymerization, high concentrations of complex monomer building blocks are required. Thermal gradients are shown to accomplish this - and retain the created RNA over long times.