Rapid analysis of titer, aggregate, and intact mass of antibody therapeutics using automated multi‐dimensional liquid chromatography coupled with native mass spectroscopy

Monoclonal antibodies are tetrameric complex proteins, primarily produced using mammalian cell culture. Attributes such as titer, aggregates, and intact mass analysis are monitored during process development/optimization. In the present study, a novel workflow such that the Protein‐A affinity chroma...

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Bibliographic Details
Published in:Journal of separation science 2023-07, Vol.46 (13), p.e2201050-n/a
Main Authors: Savane, Tushar Sharad, Kumar, Sunil, Rathore, Anurag S.
Format: Article
Language:English
Subjects:
Affinity
affinity chromatography
antibodies
Buffers
cell culture
Chromatography
gel chromatography
industry
Ions
Liquid chromatography
mammals
Mass spectrometry
Monoclonal antibodies
native mass spectrometry
Optimization
product quality
Proteins
protein‐A affinity chromatography
Quality management
rapid methods
risk
sample size
separation
Size exclusion chromatography
therapeutics
two‐dimensional liquid chromatography
Workflow
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