Decay of Thermal Resistance Following Acute Heating Is Independent of the G1- to S-Phase Transition

The phenomena of heat-induced G1 delay and thermal resistance were compared in synchronous populations of CHO cells. Mildly toxic induction doses of 5 min (Single cell survival, (SCS) = 0.90 ± 0.06) and 10 min (SCS = 0.69 ± 0.12) at 45°C resulted in G1 delays of 4.3 and 11.3 h, respectively. Thermal...

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Bibliographic Details
Published in:Radiation research 1986-07, Vol.107 (1), p.143-146
Main Author: Coss, Ronald A.
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Animals
Cell cycle
Cell Line
Cell Survival
CHO cells
Correspondence
Cricetinae
Cricetulus
Dosage
Heat shock proteins
Heat tolerance
Hot Temperature
Hyperthermia
In Vitro Techniques
Interphase
Radioactive decay
Radiotherapy
Thermal resistance
Time Factors
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Summary:The phenomena of heat-induced G1 delay and thermal resistance were compared in synchronous populations of CHO cells. Mildly toxic induction doses of 5 min (Single cell survival, (SCS) = 0.90 ± 0.06) and 10 min (SCS = 0.69 ± 0.12) at 45°C resulted in G1 delays of 4.3 and 11.3 h, respectively. Thermal resistance was tested (30 min, 45°C) for up to 32-92 h following the induction dose. Thermal resistance did not start to decay prior to 26 h following the induction dose. These data confirm reports by R. R. Read, M. H. Fox, and J. S. Bedford [Radiat. Res. 98, 491-505 (1984)] and G. L. Rice, J. W. Gray, P. N. Dean, and W. C. Dewey [Cancer Res. 44, 2368-2376 (1984)] that acutely heated G1 populations of CHO cells progress into S phase without a concurrent loss of thermal resistance, using 45°C induction doses even less toxic than used by other workers.
ISSN:0033-7587
1938-5404
DOI:10.2307/3576858