Loss of the auxiliary α 2 δ 1 voltage-sensitive calcium channel subunit impairs bone formation and anabolic responses to mechanical loading

• Published in: JBMR plus 2024-02, Vol.8 (2), p.ziad008
• Main Authors: Kelly, Madison M, Sharma, Karan, Wright, Christian S, Yi, Xin, Reyes Fernandez, Perla C, Gegg, Aaron T, Gorrell, Taylor A, Noonan, Megan L, Baghdady, Ahmed, Sieger, Jacob A, Dolphin, Annette C, Warden, Stuart J, Deosthale, Padmini, Plotkin, Lilian I, Sankar, Uma, Hum, Julia M, Robling, Alexander G, Farach-Carson, Mary C, Thompson, William R
• Format: Article
• Language: English
• ISSN: 2473-4039

Voltage-sensitive calcium channels (VSCCs) influence bone structure and function, including anabolic responses to mechanical loading. While the pore-forming (α ) subunit of VSCCs allows Ca influx, auxiliary subunits regulate the biophysical properties of the pore. The α δ subunit influences gating kinetics of the α pore and enables mechanically induced signaling in osteocytes; however, the skeletal function of α δ in vivo remains unknown. In this work, we examined the skeletal consequences of deleting , the gene encoding α δ . Dual-energy X-ray absorptiometry and microcomputed tomography imaging demonstrated that deletion of α δ diminished bone mineral content and density in both male and female C57BL/6 mice. Structural differences manifested in both trabecular and cortical bone for males, while the absence of α δ affected only cortical bone in female mice. Deletion of α δ impaired skeletal mechanical properties in both sexes, as measured by three-point bending to failure. While no changes in osteoblast number or activity were found for either sex, male mice displayed a significant increase in osteoclast number, accompanied by increased eroded bone surface and upregulation of genes that regulate osteoclast differentiation. Deletion of α δ also rendered the skeleton insensitive to exogenous mechanical loading in males. While previous work demonstrates that VSCCs are essential for anabolic responses to mechanical loading, the mechanism by which these channels sense and respond to force remained unclear. Our data demonstrate that the α δ auxiliary VSCC subunit functions to maintain baseline bone mass and strength through regulation of osteoclast activity and also provides skeletal mechanotransduction in male mice. These data reveal a molecular player in our understanding of the mechanisms by which VSCCs influence skeletal adaptation.