Dual amplification for PEC ultrasensitive aptasensing of biomarker HER-2 based on Z-scheme UiO-66/CdIn 2 S 4 heterojunction and flower-like PtPdCu nanozyme

• Published in: Talanta (Oxford) 2024-04, Vol.274, p.126034
• Main Authors: Ai, Qing-Ying, Xu, Ben-Fang, Xu, Fan, Wang, Ai-Jun, Mei, Li-Ping, Wu, Liang, Song, Pei, Feng, Jiu-Ju
• Format: Article
• Language: English
• ISSN: 1873-3573
• DOI: 10.1016/j.talanta.2024.126034

As an important prognostic indicator in breast cancer, human epithelial growth factor receptor-2 (HER-2) is of importance for assessing prognosis of breast cancer patients, whose accurate and facile analysis are imperative in clinical diagnosis and treatment. Herein, photoactive Z-scheme UiO-66/CdIn S heterojunction was constructed by a hydrothermal method, whose optical property and photoactivity were critically investigated by a range of techniques, combined by elucidating the interfacial charge transfer mechanism. Meanwhile, PtPdCu nanoflowers (NFs) were fabricated by a simple aqueous wet-chemical method, whose peroxidase (POD)-mimicking catalytic activity was scrutinized by representative tetramethylbenzidine (TMB) oxidation in H O system. Taken together, the UiO-66/CdIn S based photoelectrochemical (PEC) aptasensor was established for quantitative analysis of HER-2, where the detection signals were further magnified through catalytic precipitation reaction towards 4-chloro-1-naphthol (4-CN) oxidation (assisted by the PtPdCu NFs nanozyme). The PEC aptasensor presented a broader linear range within 0.1 pg mL -0.1 μg mL and a lower limit of detection of 0.07 pg mL . This work developed a new PEC aptasensor for ultrasensitive determination of HER-2, holding substantial promise for clinical diagnostics.