Dynamic remodeling of TRPC5 channel-caveolin-1-eNOS protein assembly potentiates the positive feedback interaction between Ca 2+ and NO signals

• Published in: The Journal of biological chemistry 2024-09, Vol.300 (9), p.107705
• Main Authors: Sakaguchi, Reiko, Takahashi, Nobuaki, Yoshida, Takashi, Ogawa, Nozomi, Ueda, Yoshifumi, Hamano, Satoshi, Yamaguchi, Kaori, Sawamura, Seishiro, Yamamoto, Shinichiro, Hara, Yuji, Kawamoto, Tomoya, Suzuki, Ryosuke, Nakao, Akito, Mori, Masayuki X, Furukawa, Tetsushi, Shimizu, Shunichi, Inoue, Ryuji, Mori, Yasuo
• Format: Article
• Language: English
• Subjects: Animals; Calcium - metabolism; Calcium Signaling - physiology; Caveolin 1 - genetics; Caveolin 1 - metabolism; Endothelial Cells - metabolism; Feedback, Physiological; HEK293 Cells; Humans; Male; Nitric Oxide - metabolism; Nitric Oxide Synthase Type III - metabolism; Rats; Signal Transduction; TRPC Cation Channels - genetics; TRPC Cation Channels - metabolism
• ISSN: 1083-351X

The cell signaling molecules nitric oxide (NO) and Ca regulate diverse biological processes through their closely coordinated activities directed by signaling protein complexes. However, it remains unclear how dynamically the multicomponent protein assemblies behave within the signaling complexes upon the interplay between NO and Ca signals. Here we demonstrate that TRPC5 channels activated by the stimulation of G-protein-coupled ATP receptors mediate Ca influx, that triggers NO production from endothelial NO synthase (eNOS), inducing secondary activation of TRPC5 via cysteine S-nitrosylation and eNOS in vascular endothelial cells. Mutations in the caveolin-1-binding domains of TRPC5 disrupt its association with caveolin-1 and impair Ca influx and NO production, suggesting that caveolin-1 serves primarily as the scaffold for TRPC5 and eNOS to assemble into the signal complex. Interestingly, during ATP receptor activation, eNOS is dissociated from caveolin-1 and in turn directly associates with TRPC5, which accumulates at the plasma membrane dependently on Ca influx and calmodulin. This protein reassembly likely results in a relief of eNOS from the inhibitory action of caveolin-1 and an enhanced TRPC5 S-nitrosylation by eNOS localized in the proximity, thereby facilitating the secondary activation of Ca influx and NO production. In isolated rat aorta, vasodilation induced by acetylcholine was significantly suppressed by the TRPC5 inhibitor AC1903. Thus, our study provides evidence that dynamic remodeling of the protein assemblies among TRPC5, eNOS, caveolin-1, and calmodulin determines the ensemble of Ca mobilization and NO production in vascular endothelial cells.