Integrative structural analysis of Pseudomonas phage DEV reveals a genome ejection motor

DEV is an obligatory lytic Pseudomonas phage of the N4-like genus, recently reclassified as Schitoviridae . The DEV genome encodes 91 ORFs, including a 3398 amino acid virion-associated RNA polymerase (vRNAP). Here, we describe the complete architecture of DEV, determined using a combination of cryo...

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Bibliographic Details
Published in:Nature communications 2024-10, Vol.15 (1), p.8482-20, Article 8482
Main Authors: Lokareddy, Ravi K., Hou, Chun-Feng David, Forti, Francesca, Iglesias, Stephano M., Li, Fenglin, Pavlenok, Mikhail, Horner, David S., Niederweis, Michael, Briani, Federica, Cingolani, Gino
Format: Article
Language:English
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Amino acids
Capsid - metabolism
Capsid - ultrastructure
Capsid Proteins - chemistry
Capsid Proteins - genetics
Capsid Proteins - metabolism
Cryoelectron Microscopy
DNA-directed RNA polymerase
DNA-Directed RNA Polymerases - genetics
DNA-Directed RNA Polymerases - metabolism
Ejection
Electron microscopes
Electron microscopy
Fibers
Genome, Viral - genetics
Genomes
Genomic analysis
Humanities and Social Sciences
Lipopolysaccharides
Microscopy
multidisciplinary
Open Reading Frames - genetics
Operon - genetics
Phages
Pseudomonas
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - virology
Pseudomonas Phages - genetics
Pseudomonas Phages - ultrastructure
Ribonucleic acid
RNA
RNA polymerase
Science
Science (multidisciplinary)
Structural analysis
Tail fiber protein
Transmission electron microscopy
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - metabolism
Virion - genetics
Virion - ultrastructure
Virions
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