Detection of Cellular Immunity to Tumor Antigens of a Guinea Pig Hepatoma by Inhibition of Macrophage Migration

Cellular immunity to a transplantable diethylnitrosamine-induced strain-2 guinea pig hepatoma was detected in vitro by inhibition of macrophage migration. Addition of 10% line-1 tumor cells to peritoneal exudate cells from strain-2 guinea pigs immunized intradermally against line-1 hepatoma inhibite...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1972-02, Vol.48 (2), p.541-549
Main Authors: Churchill, Winthrop H., Zbar, Berton, Belli, James A., David, John R.
Format: Article
Language:English
Subjects:
Animals
Antigens, Neoplasm
Carcinogens
Carcinoma, Hepatocellular - chemically induced
Carcinoma, Hepatocellular - immunology
Cell Migration Inhibition
Epitopes
Freezing
Guinea Pigs
Immunity, Cellular
Immunization
Liver Neoplasms - chemically induced
Liver Neoplasms - immunology
Lymphocytes - immunology
Macrophages
Male
Neoplasm Transplantation
Neoplasms, Experimental - chemically induced
Neoplasms, Experimental - immunology
Nitrosamines
Radiation Chimera
Transplantation, Homologous
Trypsin - pharmacology
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Summary:Cellular immunity to a transplantable diethylnitrosamine-induced strain-2 guinea pig hepatoma was detected in vitro by inhibition of macrophage migration. Addition of 10% line-1 tumor cells to peritoneal exudate cells from strain-2 guinea pigs immunized intradermally against line-1 hepatoma inhibited macrophage migration. The average migration index was 41% in comparison to 107% for migration of control peritoneal exudate cells. Inhibition was produced only by the immunizing tumor and not by antigenically unrelated tumor cells or normal spleen cells. Lymphocytes, purified from peritoneal exudate cells harvested from strain-2 guinea pigs, immunized by intradermal injection of living syngeneic tumor cells without any adjuvant, produced migration inhibitory factor (MIF) in response to tumor cell antigens. Tumor cells treated by X radiation, trypsinization, or storage in liquid nitrogen after slow freezing retained their capacity to stimulated MIF production by sensitized lymphocytes.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/48.2.541