Suppression of tumor-specific cell-mediated cytotoxicity by immunoregulatory alpha-globulin and by immunoregulatory alpha-globulin-like peptides from cancer patients

The suppression of tumor-specific cell-mediated cytotoxicity by human immunoregulatory alpha-globulin (IRA), by a peptide fraction derived from IRA, and by IRA-like peptides from the serum of cancer patients was studied in a syngeneic murine tumor-host system. Splenic lymphocytes from tumor-immunize...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1977-01, Vol.37 (9), p.3022
Main Authors: Wang, B S, Badger, A M, Nimberg, R R, Cooperband, S R, Schmid, K, Mannick, J A
Format: Article
Language:English
Subjects:
Alpha-Globulins - immunology
Alpha-Globulins - pharmacology
Animals
Antigens, Neoplasm
Cytotoxicity Tests, Immunologic
Female
Hemolytic Plaque Technique
Humans
Immunity, Cellular
Immunosuppression
In Vitro Techniques
Mice
Mice, Inbred C3H
Neoplasms - immunology
Peptides - immunology
T-Lymphocytes - immunology
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Summary:The suppression of tumor-specific cell-mediated cytotoxicity by human immunoregulatory alpha-globulin (IRA), by a peptide fraction derived from IRA, and by IRA-like peptides from the serum of cancer patients was studied in a syngeneic murine tumor-host system. Splenic lymphocytes from tumor-immunized mice were cytotoxic specific tumor cells in vitro as measured by the [125]-iododeoxyuridine release microcytotoxicity assay. However, this effect was significantly depressed if 1.25 to 5 mg of IRA per ml were added to the cultures. Pooled lyophilized normal human serum protein was inactive. IRA peptide and IRA-like peptide fractions from cancer patients were also highly suppressive of cell-mediated cytotoxicity at much lower concentrations (0.05 to 0.5 mg/ml). Control human serum peptide, which failed to inhibit the induction of hemolytic plaque-forming cells in sheep erythrocyte-injected mice, had no effect on cell-mediated cytotoxicity. IRA and IRA-like peptide fractions were not cytotoxic to the effector lymphocytes or to the target cells at the concentrations used.
ISSN:0008-5472