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Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase
The gene encoding extracellular xylanase (xynA) was amplified as a 770 bp DNA fragment from Bacillus subtilis 168 chromosomal DNA by PCR. The genes encoding endo-beta-1,4glucanase (eglS) and endo-beta-1,3-1,4 glucanase (bglS) were isolated from a genomic library of B. subtilis 168. The sequences of...
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Published in: | Microbiology (Society for General Microbiology) 1995-02, Vol.141, p.281-290 |
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creator | Wolf, M Geczi, A Simon, O Borriss, R |
description | The gene encoding extracellular xylanase (xynA) was amplified as a 770 bp DNA fragment from Bacillus subtilis 168 chromosomal DNA by PCR. The genes encoding endo-beta-1,4glucanase (eglS) and endo-beta-1,3-1,4 glucanase (bglS) were isolated from a genomic library of B. subtilis 168. The sequences of xynA and eglS were identical to those of the xylanase and cellulase genes from B. subtilis PAP115. Integrative plasmids containing DNA fragments with deletions in the coding region of the genes were constructed and used to replace the chromosomal eglS, bglS and xynA genes of B. subtilis 168. Strains without any detectable activity against xylan (Xyn-), carboxymethylcellulose (Egl-) or mixed linked beta-1,3-1,4-glucan (Egl- Bgl-) were obtained. The genes were mapped at 170 degrees (eglS), 175 degrees (xynA) and 340 degrees (bglS) on the B. subtilis chromosome. |
doi_str_mv | 10.1099/13500872-141-2-281 |
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The genes encoding endo-beta-1,4glucanase (eglS) and endo-beta-1,3-1,4 glucanase (bglS) were isolated from a genomic library of B. subtilis 168. The sequences of xynA and eglS were identical to those of the xylanase and cellulase genes from B. subtilis PAP115. Integrative plasmids containing DNA fragments with deletions in the coding region of the genes were constructed and used to replace the chromosomal eglS, bglS and xynA genes of B. subtilis 168. Strains without any detectable activity against xylan (Xyn-), carboxymethylcellulose (Egl-) or mixed linked beta-1,3-1,4-glucan (Egl- Bgl-) were obtained. The genes were mapped at 170 degrees (eglS), 175 degrees (xynA) and 340 degrees (bglS) on the B. subtilis chromosome.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/13500872-141-2-281</identifier><identifier>PMID: 7704256</identifier><language>eng</language><publisher>Reading: Society for General Microbiology</publisher><subject>Bacillus subtilis - enzymology ; Bacillus subtilis - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Biological Sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Cellulase - deficiency ; Cellulase - genetics ; Cloning, Molecular ; Endo-1,4-beta Xylanases ; Fundamental and applied biological sciences. Psychology ; genbank/z29076 ; genbank/z34519 ; genbank/z46862 ; Genes, Bacterial - genetics ; Genetic Complementation Test ; Genetic Linkage ; Genetics ; Glucans - metabolism ; Glycoside Hydrolases - deficiency ; Glycoside Hydrolases - genetics ; Hydrolysis ; Microbiology ; Mission oriented research ; Molecular Sequence Data ; Mutagenesis ; Restriction Mapping ; Sequence Analysis, DNA ; Xylans - metabolism ; Xylosidases - deficiency ; Xylosidases - genetics</subject><ispartof>Microbiology (Society for General Microbiology), 1995-02, Vol.141, p.281-290</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3407634$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7704256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolf, M</creatorcontrib><creatorcontrib>Geczi, A</creatorcontrib><creatorcontrib>Simon, O</creatorcontrib><creatorcontrib>Borriss, R</creatorcontrib><title>Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>The gene encoding extracellular xylanase (xynA) was amplified as a 770 bp DNA fragment from Bacillus subtilis 168 chromosomal DNA by PCR. The genes encoding endo-beta-1,4glucanase (eglS) and endo-beta-1,3-1,4 glucanase (bglS) were isolated from a genomic library of B. subtilis 168. The sequences of xynA and eglS were identical to those of the xylanase and cellulase genes from B. subtilis PAP115. Integrative plasmids containing DNA fragments with deletions in the coding region of the genes were constructed and used to replace the chromosomal eglS, bglS and xynA genes of B. subtilis 168. Strains without any detectable activity against xylan (Xyn-), carboxymethylcellulose (Egl-) or mixed linked beta-1,3-1,4-glucan (Egl- Bgl-) were obtained. The genes were mapped at 170 degrees (eglS), 175 degrees (xynA) and 340 degrees (bglS) on the B. subtilis chromosome.</description><subject>Bacillus subtilis - enzymology</subject><subject>Bacillus subtilis - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological Sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Cellulase - deficiency</subject><subject>Cellulase - genetics</subject><subject>Cloning, Molecular</subject><subject>Endo-1,4-beta Xylanases</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genbank/z29076</subject><subject>genbank/z34519</subject><subject>genbank/z46862</subject><subject>Genes, Bacterial - genetics</subject><subject>Genetic Complementation Test</subject><subject>Genetic Linkage</subject><subject>Genetics</subject><subject>Glucans - metabolism</subject><subject>Glycoside Hydrolases - deficiency</subject><subject>Glycoside Hydrolases - genetics</subject><subject>Hydrolysis</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>Restriction Mapping</subject><subject>Sequence Analysis, DNA</subject><subject>Xylans - metabolism</subject><subject>Xylosidases - deficiency</subject><subject>Xylosidases - genetics</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNo9kEtr3TAQhUVpyav9A4USLbKMGo0ettxdG_IoBLJIsr6MJfleFV35YtkQ52_1D0ZOLlnNHM53DsMQ8h34T-BNcwFSc25qwUABE0wY-ESOQFWaCW7457IXgC3EITnO-R_nxeRwQA7qmiuhqyPy_8Ynn6lPtnchrenzHDFRTI62fkS2jpMtejO7oY9zXgifXuZtiYRE_6ANMU6Z5qkdQwz5F7UbHNCOfggvOIY-ndMt7nZLbum0fcrjMNnFoX1Hi8CQMnW-Czb4NC6tMdiNT5j9ObW-1MeyvqXfbiviK_nSYcz-236ekKfrq8fLW3Z3f_P38vcd66BWI2s0WgDhBYJqDVbWq6bVRohKV8aAACcVVKYF5QSXktfgrAHduApRCVTyhPx4791N7da71W4IWxzm1f55xT_b-5gtxm7AZEP-wKTidSWXmtN3rMN-heuhIE8PgoPkoIWWNZev0Y6LAA</recordid><startdate>199502</startdate><enddate>199502</enddate><creator>Wolf, M</creator><creator>Geczi, A</creator><creator>Simon, O</creator><creator>Borriss, R</creator><general>Society for General Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>199502</creationdate><title>Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase</title><author>Wolf, M ; Geczi, A ; Simon, O ; Borriss, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f174t-95ac112e2a14b8a6ce49b582265688121d34168b14d2033071dc8159d6aa42a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Bacillus subtilis - enzymology</topic><topic>Bacillus subtilis - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biological Sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Cellulase - deficiency</topic><topic>Cellulase - genetics</topic><topic>Cloning, Molecular</topic><topic>Endo-1,4-beta Xylanases</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genbank/z29076</topic><topic>genbank/z34519</topic><topic>genbank/z46862</topic><topic>Genes, Bacterial - genetics</topic><topic>Genetic Complementation Test</topic><topic>Genetic Linkage</topic><topic>Genetics</topic><topic>Glucans - metabolism</topic><topic>Glycoside Hydrolases - deficiency</topic><topic>Glycoside Hydrolases - genetics</topic><topic>Hydrolysis</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis</topic><topic>Restriction Mapping</topic><topic>Sequence Analysis, DNA</topic><topic>Xylans - metabolism</topic><topic>Xylosidases - deficiency</topic><topic>Xylosidases - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolf, M</creatorcontrib><creatorcontrib>Geczi, A</creatorcontrib><creatorcontrib>Simon, O</creatorcontrib><creatorcontrib>Borriss, R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolf, M</au><au>Geczi, A</au><au>Simon, O</au><au>Borriss, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>1995-02</date><risdate>1995</risdate><volume>141</volume><spage>281</spage><epage>290</epage><pages>281-290</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>The gene encoding extracellular xylanase (xynA) was amplified as a 770 bp DNA fragment from Bacillus subtilis 168 chromosomal DNA by PCR. The genes encoding endo-beta-1,4glucanase (eglS) and endo-beta-1,3-1,4 glucanase (bglS) were isolated from a genomic library of B. subtilis 168. The sequences of xynA and eglS were identical to those of the xylanase and cellulase genes from B. subtilis PAP115. Integrative plasmids containing DNA fragments with deletions in the coding region of the genes were constructed and used to replace the chromosomal eglS, bglS and xynA genes of B. subtilis 168. Strains without any detectable activity against xylan (Xyn-), carboxymethylcellulose (Egl-) or mixed linked beta-1,3-1,4-glucan (Egl- Bgl-) were obtained. The genes were mapped at 170 degrees (eglS), 175 degrees (xynA) and 340 degrees (bglS) on the B. subtilis chromosome.</abstract><cop>Reading</cop><pub>Society for General Microbiology</pub><pmid>7704256</pmid><doi>10.1099/13500872-141-2-281</doi><tpages>10</tpages></addata></record> |
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subjects | Bacillus subtilis - enzymology Bacillus subtilis - genetics Bacteriology Base Sequence Biological and medical sciences Biological Sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Cellulase - deficiency Cellulase - genetics Cloning, Molecular Endo-1,4-beta Xylanases Fundamental and applied biological sciences. Psychology genbank/z29076 genbank/z34519 genbank/z46862 Genes, Bacterial - genetics Genetic Complementation Test Genetic Linkage Genetics Glucans - metabolism Glycoside Hydrolases - deficiency Glycoside Hydrolases - genetics Hydrolysis Microbiology Mission oriented research Molecular Sequence Data Mutagenesis Restriction Mapping Sequence Analysis, DNA Xylans - metabolism Xylosidases - deficiency Xylosidases - genetics |
title | Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase |
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