Testing bone implants in cell lines and human osteoblasts

Two different types of cell-line and fresh human osteoblasts, cultured from cancellous bone grafts from the iliac crest, were used for the study. Three different biomaterials were compared regarding biocompatibility: titanium, steel and hydroxyapatite. The cells were fibroblast cell line (3T3), &quo...

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Bibliographic Details
Published in:Der Unfallchirurg 1997-01, Vol.100 (1), p.39
Main Authors: Trentz, O A, Zellweger, R, Amgwerd, M G, Uhlschmid, G K
Format: Article
Language:ger
Subjects:
Bone Plates
Bone Substitutes
Cell Line
Durapatite
Fracture Fixation, Internal - instrumentation
Humans
Materials Testing
Osteoblasts - cytology
Prostheses and Implants
Steel
Titanium
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Summary:Two different types of cell-line and fresh human osteoblasts, cultured from cancellous bone grafts from the iliac crest, were used for the study. Three different biomaterials were compared regarding biocompatibility: titanium, steel and hydroxyapatite. The cells were fibroblast cell line (3T3), "osteoblast-like" cell line (MC3T3-E1), and fresh human osteoblasts (HOB) which we cultured in our laboratory. 5 x 10(4) cells of each type were seeded on the three different bone implants. All experiments were performed in triplicate. Cell proliferation and alkaline phosphatase activity were determined 24 and 72 h after the cells were plated on the biomaterials. Human osteoblast growth was better on titanium than on steel and hydroxyapatite. The most remarkable observation was the continuously decreasing alkaline phosphatase activity (ALP) of "osteoblast-like" cells (MC3T3-E1) and human osteoblasts (HOB) on hydroxyapatite. In conclusion, our in vitro observations suggest that the best cell/material interactions were with human osteoblasts (HOB) and titanium.
ISSN:0177-5537